Without an adequate DNA extraction protocol, the identification of Plasmodium species in whole mosquitoes by PCR is difficult because of the presence of reaction inhibitors from the insects. In this study, eight DNA extraction protocols were tested, from which a chelex-based protocol was selected. Then a semi-nested multiplex PCR technique that detects and distinguishes among the four human Pla...

We examined polymorphism of the TCTA tetranucleotide sequence in the 3rd intron of the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene in the Han population of Ningxia Province in China. We also looked for a possible relationship between STR polymorphism in the 3rd intron of the HPRT gene and primary hyperuricemia. We used Chelex-100 to extract DNA, then PCR, PAGE and silver st...

A flow injection photometric method for the sequential determination of zinc and copper in mixtures was developed based on the variation of the stability of the chromogenic complexes between the analytes and the reagent zincon with pH. At pH 5.0 only the Cu-zincon complex exists, whereas at pH 9.0 the copper and zinc chelates co-exist. A three-channel manifold was implemented containing two alt...

Guanylate cyclase in neuroblastoma N1E 115 cells was readily solubilized upon homogenization of the cells with hypotonic buffer. When the supernatant was passed through cation exchangers such as a Chelex 100 Na+ column, the guanylate cyclase activity in the effluent fraction decreased to 4-6% of the original supernatant. The addition of the acid extract of neuroblastoma cells or rat tissues to ...

Cation-exchange fast protein liquid chromatography – electrothermal atomic absorption spectrometry (FPLC – ETAAS) was applied for the speciation of trace amounts of aluminium. An aqueous 8 mol dm–3 NH4NO3 linear gradient elution in 10 minutes enabled separation of positively charged monomeric aluminium species in aqueous solution. Separated aluminium species were collected in 0.5 cm3 fractions ...

AIM To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations. METHODS DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2...

13 The dynamic technique of diffusive gradients in thin films (DGT), that measures metal 14 speciation in situ, has found wide environmental application. Simple interpretation of the 15 metal accumulation in terms of a solution concentration has assumed that trace metals do 16 not penetrate beyond the surface of the binding layer, but penetration, although 17 theoretically discussed has not yet...

This paper describes the equilibrium behaviour of a wastewater resembling that produced during semiconductor and printed circuit board manufacture when contacted with a chelating ion exchange resin (Chelex-100; Bio-Rad Laboratories). Distribution coefficients (solid phase metal concentrations/soluble metal concentration) were calculated, and it was determined that the order of affinity was Cu >...

The influence of Zn on the expression of the apolipoprotein A-I (apoA-I) gene in Hep G2 cells was examined. Zn depletion was achieved with a low-Zn (ZD) medium prepared from Zn-free growth medium (Opti), a ZD medium containing Chelex 100-extracted fetal bovine serum (CHE), and a medium containing chelator 1,10-phenanthroline (OP). Compared with those for their respective controls, cellular Zn l...

OBJECTIVES This preliminary study was carried out to determine the allele frequencies and forensic efficiency parameters for the short tandem repeat loci CSF1PO, TPOX, THO1, D16S539, D7S820, D13S317, vWA, FESFPS and F13B in a test sample population of Sri Lankans. DESIGN Test samples were obtained from 305 non-related individuals originating from all 9 provinces of Sri Lanka. DNA was extracte...