Cellulose synthesis, but not its degradation, is generally thought to be required for plant cell growth. In this work, we cloned a dinoflagellate cellulase gene, dCel1, whose activities increased significantly in G2/M phase, in agreement with the significant drop of cellulose content reported previously. Cellulase inhibitors not only caused a delay in cell cycle progression at both the G1 and G...

Sophorose (2-O-beta-glucopyranosyl-D-glucose) induces carboxymethyl cellulase in Trichoderma reesei QM6a mycelium with 1.5 to 2 h. The induction response to sophorose concentration, although complicated by the metabolism of sophorose, shows saturation kinetics. Most of the cellulase appears after most of the sophorose has been taken up, but the presence of an inducer is required to maintain cel...

In optimization of cellulase production by different pH, substrates, nitrogen, carbon sources were detected from penicillium citrinum micro-fungal species. The maximum cellulase activity was recorded in the range of pH5 to 6.5 and wheat bran (91 U/ml) and rice bran (81 U/ml) is the best raw substrate for the production of cellulase. Estimation of extracellular protein was recorded in different ...

TiO2 photocatalyst was prepared using cellulase as a biotemplate through hydrothermal process. The as-prepared catalyst characterized based on physicochemical techniques and used to remove As(III) from contaminated water. templated (TiO2-cellulase) had large specific surface area, which reached 166.6 m2·g–1. It also strong oxidation ability and, thus, behaved well in both photocatalytic of dark...

Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification. Nevertheless, the promoting mechanisms not fully understood there are a number of controversial opinions existed. All attention has paid to interactions between respective additive substrate. However, rarely additives enzymes (cel...

In Trichoderma reesei light is an important factor in the regulation of glycoside hydrolase gene expression. We therefore investigated the influence of different light intensities on cellulase activity and protein secretion. Differentially secreted proteins in light and darkness as identified by mass spectrometry included members of different glycoside hydrolase families, such as CBH1, Cel3A, C...

BACKGROUND Trichoderma reesei is one of the most important fungi utilized for cellulase production. However, its cellulase system has been proven to be present in suboptimal ratio for deconstruction of lignocellulosic substrates. Although previous enzymatic optimization studies have acquired different types of in vitro synthetic mixtures for efficient lignocellulose hydrolysis, production of in...

The use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4 × 10(6)  propagules ml(-1)) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill any significant number. Infested tomato roots that were inoculated with mycelia plus pectina...

Solid-state fermentation conditions were investigated for cellulase production by marine Aspergillus ZJUBE-1 using crop straw as the main material. When Aspergillus ZJUBE-1 was grown under the condition: ratio of rice straw to wheat bran was 1:1, the ratio of material to liquid was 1:2, the ratio of nitrogen and carbon was 5.6%, NaCl was 2.4~4.0%, temperature at 37 °C, the maximum CMCase activi...