This paper presents a method of particle manipulation by dielectrophoresis (DEP) and immobilization using photo‐crosslinkable resin inside microfluidic devices. High speed particle manipulation, including patterning and concentration control by DEP was demonstrated. Immovable and movable microstructures embedding particles were fabricated on‐ chip. Several microelec...

objective(s): enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. in this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. materials and methods:  the process consists of the ...

Planarians are an important model organism for regeneration and stem cell research. A complete understanding of stem cell and regeneration dynamics in these animals requires time-lapse imaging in vivo, which has been difficult to achieve due to a lack of tissue-specific markers and the strong negative phototaxis of planarians. We have developed the Planarian Immobilization Chip (PIC) for rapid,...

VELICHKOVA, E., M. YORDANOVA, M. BADALOVA, D. NIKOLOVA and Y. EVSTATIEVA, 2014. Adherence of Aspergillus awamori K-1 and Trichoderma viride SL-45 on loofa sponge for production of hydrolases. Bulg. J. Agric. Sci., Supplement 1: 97–99 In nature, fi lamentous fungi of the genus Aspergillus and Trichoderma are able to secrete a wide range of different enzymes into their environment. In recent year...

PURPOSE Sperm immobilization factor isolated from Staphylococcus aureus immobilizes human spermatozoa as well as motile bacteria, showing that sperm immobilization factor receptor might be shared by bacteria and human spermatozoa. Thus, we sought to identify a common sperm immobilization factor binding receptor on spermatozoa and bacteria. MATERIALS AND METHODS Sperm immobilization factor was...

Effect of immobilization stress on the rat colonic temperature and metabolism was studied in a warm environment of 25 degrees C. Immobilization for 3 hr caused hyperthermia accompanied by increased oxygen consumption (VO2) in the warm controls. The hyperthermic effect of immobilization was accelerated after 1 week repetition of daily immobilization and it was lessened after 2 to 4 week repetiti...

Contact inhibition of phagocytosis was found to be characteristic for epithelial sheets formed in cultures by several cell types: normal and transformed mouse kidney cells, and differentiated mouse hepatoma cells. In these sheets most central cells surrounded by other cells had very low phagocytic activity. In contrast, marginal cells having a free edge were able to perform an active phagocytos...

OBJECTIVES To investigate alteration of bone and muscle gene expression at different time points during 3 weeks of botulinum toxin (BTX) induced immobilization and how this correlate with conventional analysis of bone and muscle. METHODS Thirty-five 16-week-old female C57BL/6-mice were investigated; 15 were injected with BTX, 15 served as age-matched controls, and 5 as baseline. 5 BTX-injecte...

Bacteria have evolved complex, highly-coordinated, multi-component cellular engines to achieve high degrees of efficiency, accuracy, adaptability, and redundancy. Super-resolution fluorescence microscopy methods are ideally suited to investigate the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. These techniques require the long-term stabili...

When force is applied to cell-matrix adhesion complexes, they respond by growing larger and stronger. It emerges that strengthening involves transient motion of the transmembrane integrin receptors and their eventual immobilization to the extracellular matrix.