نتایج جستجو برای: cell cultivation
تعداد نتایج: 1712921 فیلتر نتایج به سال:
Due to its vital importance in the supply of cellular pathways with energy and precursors, glycolysis has been studied for several decades regarding its capacity and regulation. For a systems-level understanding of the Madin-Darby canine kidney (MDCK) cell metabolism, we couple a segregated cell growth model published earlier with a structured model of glycolysis, which is based on relatively s...
AIM To compare biological properties of primary tumor cells isolated from malignant effusion of cancer patients with the same cells of permanent lines established during their long-term cultivation in vitro and to assess the impact of phenotypic conversion that was caused by changes in their microenvironment on their behavioral characteristics. MATERIALS AND METHODS The study was performed on...
Ecthyma contagiosum is a viral disease of sheep and goats, which is caused by Parapoxvirus. Primary cell cultures of lamb testis, lamb kidney, rabbit kidney and permanent cell lines: foetal sheep kidney (FSK), sheep foetal thymus (SFT), sheep foetal muscle (SFM), sheep plexus choroideus (SPC), bovine trachea (BTR), embryonic bovine trachea (EBTR), Madin Darby bovine kidney (MDBK) and baby hamst...
During last years, alpha-ketoglutarate (AKG), an important intermediate in the Krebs cycle, has been intensively studied as a dietary supplement with stress-protective and potential antiaging effects. Here, we examined the effects of exogenous AKG on metabolic processes and survival of yeast Saccharomyces cerevisiae during long-term cultivation. Growth on AKG had no effect on the total cell num...
Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biological applications and represents an interesting model system for the screening applications due ...
This thesis focuses on the design and characterisation of miniature bioreactors and evaluates their potential as a scale-down device for microbial cultivation processes. Miniature bioreactors, such as the one detailed in this work, have been developed by many research groups and companies, and seek to increase throughput at the early stages of bioprocess development. Power input was measured in...
امروزه با توسعه روز افزون تکنولوژی mems و کاربردهای گسترده آن در مهندسی پزشکی می توان قطعاتی را طراحی نمود که قابل کاشت (implantable ) در داخل بدن باشد. اندازه گیری مقدار قند خون یکی از زمینه هایی می باشد که با توسعه تکنولوژی memsمورد توجه بوده است. در بیماران دیابتی نوع دوم، میزان قند خون بالا می باشد و از آنجایی که با افزایش قند خون احتمال بیهوشی نیز بالا می رود، لذا اندازه گیری قند خون بصورت...
Background Human cell lines are an interesting alternative to CHO cells for the production of recombinant proteins and monoclonal antibodies, because of their ability to produce genuine human posttranslational modifications. The human cell line AGE1.HN.AAT (ProBioGen, Berlin, Germany), that originated from human neural precursor tissue, has been adapted to serum-free conditions and cultivated i...
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
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