Stem cell decline is an important cellular driver of aging-associated pathophysiology in multiple tissues. Epigenetic regulation is central to establishing and maintaining stem cell function, and emerging evidence indicates that epigenetic dysregulation contributes to the altered potential of stem cells during aging. Unlike terminally differentiated cells, the impact of epigenetic dysregulation...

The replicative lifespan (RLS) of a cell-defined as the number of cell divisions before death-has informed our understanding of the mechanisms of cellular aging. However, little is known about aging and longevity in symmetrically dividing eukaryotic cells because most prior studies have used budding yeast for RLS studies. Here, we describe a multiplexed fission yeast lifespan micro-dissector (m...

During development hematopoietic stem cells (HSCs) expand in number and persist throughout life by undergoing self-renewing divisions. Nevertheless, the hematopoietic system does not escape the negative effects of aging, suggesting that self-renewal is not complete. A fundamental issue in stem cell biology relates to such age-dependent loss of stem cell activity. Both stem cell intrinsic factor...

Given the duration of ageing in humans, cell culture studies are a promising approach to the study of human ageing. It is reasonable to assume that human ageing has, at least partly, a cellular origin. The question is how we can replicate in vitro the age-related changes that occur in human cells in vivo. In this review, widely used models for studying ageing in cell culture, such as Hayflick's...

Aging is associated with reduced organ function and increased disease incidence. Hematopoietic stem cell (HSC) aging driven by both cell intrinsic and extrinsic factors is linked to impaired HSC self-renewal and regeneration, aging-associated immune remodeling, and increased leukemia incidence. Compromised DNA damage responses and the increased production of reactive oxygen species (ROS) have b...

Fibroblasts are the main cell type in dermis and major contributors to wound healing process. During skin development, papillary fibroblasts from upper uniquely give rise specialized hair follicle-supporting like dermal papillae, which controls development cycling, as well arrector pili muscles that grant its contractile functions. Neonatal also only supportive of follicle regeneration, we have...

Cell senescence is associated with the secretion of many factors, the so-called "senescence-associated secretory phenotype", which may alter tissue microenvironment, stimulating the organism to clean up senescent cells and replace them with newly divided ones. Therefore, although no longer dividing, these cells are still metabolically active and influence the surrounding tissue. Much attention ...

Cardiovascular diseases are the leading cause of death worldwide and their occurrence is highly associated with age. However, lack knowledge in cardiac tissue aging a major roadblock devising novel therapies. Here, we studied effects cell extracellular matrix (ECM) on induced pluripotent stem (iPSC)-derived cardiomyocyte state, function, as well response to myocardial infarction (MI)-mimicking ...

The original glucocorticoid (GC) hypothesis of brain aging and Alzheimer's disease proposed that chronic exposure to GCs promotes hippocampal aging and AD. This proposition arose from a study correlating increasing plasma corticosterone with hippocampal astrocyte reactivity in aging rats. Numerous subsequent studies have found evidence consistent with this hypothesis, in animal models and in hu...