Humans lacking the CD3 subunit of the pre-TCR and TCR complexes exhibit a mild T lymphopenia, but have normal T cells. By contrast, CD3 -deficient mice are almost devoid of mature T cells due to an early block of intrathymic development at the CD4 CD8 double-negative (DN) stage. This suggests that in humans but not in mice, the highly related CD3 chain replaces CD3 during T-cell development. To...

Here, we report data concerning the discovery in adult human peripheral blood of a precursor cell population able to differentiate into CD4+CD3+ alpha beta + mature T cells. These cells, which represent 0.1-0.5% of total peripheral blood mononuclear cells (PBMC), express substantial levels of CD4, but lack CD3 surface expression. At a molecular level, they express the pre-T cell receptor alpha ...

from the TILN suspension of one individual patient within the cohort to demonstrate gating strategy. First, on the left the major lymphocyte cell gate was defined by excluding tumour cells, granulated cells, dead cells, and debris by size (FSC) and granularity (SSC). Next, in the second plot CD3 and CD56 double staining was used to exclude T (CD3+) and NKT (CD56+CD3+) cells, and to sequentially...

we have previously shown that the proliferative response of t cells to antigenic or mitogenic stimulus decreased with age and that caloric resection (cr) attenuated the age-related decline in proliferation and il-2 expression. because activation-induced apoptosis is known to regulate cell proliferation and eliminate the high number of activated cells during an immune response, it was of interes...

Objective: Malignant gliomas are primary brain tumors with excessive mortality and high resistance to chemotherapy and radiotherapy. The survival time for glioblastoma multiforme is about 6-12 months. As key pathogenetic mechanisms are recognized the massive necrosis, angiogenesis and hypoxia within the tumor, as well as the resistance to apoptosis. It is also suspected that altered immune resp...

The biologic role of each CD3 chain and their relative contribution to the signals transduced through the TCR/CD3 complex and to downstream activation events are still controversial: they may be specialized or redundant. We have immortalized peripheral blood CD4+ and CD8+ T lymphocytes from a human selective CD3 gamma deficiency using Herpesvirus saimiri. The accessibility of the mutant TCR/CD3...

Activation of T cells through the TCR is mediated by the TCR-CD3 signaling complex. Cross linking of this complex with Abs directed against CD3 leads to potent activation of T cells. However, such activation is not Ag-specific. We exploited the observation that the TCR-CD3 complex is clustered on T cells that have been activated by Ag by using anti-CD3 nanoparticles to selectively activate Ag-e...

Introduction Tumours connected with head and neck comprise about 5% of all tumours. The most frequent histological type of laryngeal carcinoma is squamous cell carcinoma. Different research projects suggest that the role of T lymphocytes might be significant in tumour development. iNKT cells are a new subpopulation of T cells and show cytotoxic activity against tumours. iNKT cells participate i...

Generation of tumor-reactive T cells in large numbers ex vivo is a requisite step in the adoptive immunotherapy of patients. We examined the immune responses of T cells derived from tumor vaccine-primed lymph nodes activated with anti-CD3 alone and with an anti-CD3/anti-CD28 combination. Nylon wool-purified CD3+ cells were isolated from vaccine-primed lymph nodes obtained from melanoma, renal c...