THE HUMAN CELL LINE CCRF-CEM was derived by Foley et al. (1965) from the peripheral blood of a patient with acute lymphoblastic leukaemia (ALL). When tested by Norman & Thompson (1977) CCRF-CEM was only partially sensitive to glucocorticoids, but Clone C7, isolated from the parental cell line, was lysed by pharmacological concentrations of glucocorticoids. The availability of a steroid-sensitiv...

The accumulation, metabolism, and retention of mercaptopurine (MP) was studied in four human neoplastic cell lines (three acute leukemia lines Molt-4, CCRF-CEM, and HL-60; and one Burkitt's lymphoma line, Wilson), each of which was sensitive to MP. Two cell lines resistant to MP (WilsonR and CCRF-CEMR) were also studied. The cell lines were incubated for 3 h in 10 microM [14C]MP and then placed...

Techniques have been developed to measure FdUMP, the active metabolite of 5-FUra; thymidylate synthetase (TMP synthase; 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45), the target enzyme for this antimetabolite; and dUMP, the substrate that competes with FdUMP for binding to TMP synthetase. As little as 0.02 pmol of FdUMP can be quantitated with a competitive ligand bindin...

PURPOSE Increasing physical activity level is a generally effective intervention goal for patients who suffer from chronic cancer-related fatigue (CCRF). However, patients are unlikely to benefit equally from these interventions, as their behavioral starting points might vary substantially. Therefore, we explored patterns of physical behavior of participants who suffer from CCRF. METHODS Base...

The effects of various antileukemic agents on DNA replication associated with the nuclear matrix were investigated in CCRF-CEM leukemia cells. Residual nuclear matrices were prepared by sequential treatment of nuclei with 1.5 M NaCl, DNase I, and Triton X-100 and contained 1-5, 10, and 37% of the total nuclear DNA, protein, and phospholipid, respectively. In control cells pulse-labeled for 45 s...

Rapid and efficient measurement of cancer cells is a major challenge in early cancer diagnosis. In the present study, a miniature multiplex chip was created for in situ detection of cancer cells by implementing a novel graphene oxide (GO)-based Förster resonance energy transfer (FRET) biosensor strategy, i.e. assaying the cell-induced fluorescence recovery from the dye-labeled aptamer/graphene ...

The release of galactosyltransferase, sialyltransferase, and several glycosidase activities into the growth media from sev eral normal and transformed cell lines was examined. Six of the seven cell lines released galactosyltransferase into their culture media. Only the human leukemia CCRF-CEM cells failed to release demonstrable galactosyltransferase activity. Release of galactosyltransferase a...

Abstract T-cell acute lymphoblastic leukemia (T-ALL) is a hardly curable disease with high relapse rate. 20 analogs were synthesized based on the structures of two kinds fungi-derived polyenylpyrrole products (rumbrin (1) and auxarconjugatin-B (2)) to suppress growth T-ALL-derived cell line CCRF-CEM tested for growth-inhibiting activity. The octatetraenylpyrrole analog gave an IC50 0.27 μM in c...

Several 2',3'-dideoxynucleosides (ddNs), agents that inhibit the replication of human immunodeficiency virus and hepatitis B virus, enter mammalian cells by simple diffusion. In this report, we show that the membrane permeation of 2',3'-dideoxyguanosine (ddG) in human erythrocytes and CCRF-CEM cells, in contrast with that of other ddNs, is transporter-mediated. Inward fluxes of ddG in both cell...

Background The intention of cancer chemotherapy is to control the growth of cancer cells using chemical agents. However, the occurrence of second malignancies has raised concerns, leading to re-evaluation of the current strategy in use for chemotherapeutic agents. Although the mechanisms involved in second malignancy remain ambiguous, therapeutic-agent-induced non-DNA targeted effects like byst...