Abstract In Pseudomonas aeruginosa , Ttg2D is the soluble periplasmic phospholipid-binding component of an ABC transport system thought to be involved in maintaining asymmetry outer membrane. Here we use crystallographic structure at 2.5 Å resolution reveal that this protein can accommodate four acyl chains. Analysis available structures orthologs shows they conform a new substrate-binding-prot...

A key to the development of lipid membrane-based devices is a fundamental understanding how molecular structure bilayer membrane influenced by type lipids used build membrane. This particularly important when proteins are included in these since precise environment affects ability incorporate and their functionality. Here, we neutron reflectometry investigate tethered membranes characterize inc...

Salt-induced fluid lamellar (L alpha) to inverted hexagonal (HII) phase transitions have been studied in diphosphatidylglycerols (cardiolipins) with different acyl chain compositions, using 31P nuclear magnetic resonance (NMR) spectroscopy. Cardiolipins with four myristoyl chains, tetramyristoyl cardiolipin (TMCL), and with four oleoyl chains, tetraoleoyl cardiolipin (TOCL), were synthesized ch...

In 1941 Pangborn (1) reported the isolation of a complex phosphatidic acid from beef heart and suggested the name cardiolipin for it. The presence of this phospholipide seems to be essential for the complementbinding activity of beef heart extracts with sera of syphilitic patients. On hydrolysis (2) with ethanolic potassium hydroxide at room temperature the phosphatidic acid yielded, in additio...

1. The influence of Ca2+ on the polymorphic phase behaviour of cardiolipin has been investigated employing 31P NMR and freeze-fracture techniques. The close correlation between the results obtained here and previous X-ray studies (Rand, R.P. and Sengupta, S. (1972) Biochim. Biophys. Acta 255, 484--492) confirms 31P NMR as a useful analytical procedure for investigating the polymorphic phase beh...