To investigate the potential for the utilization of cotton stalk, ammonia hydrothermal treatment was applied to fractionate the samples into aqueous ammonia-soluble and ammonia-insoluble portions. The ammonia-soluble portion was purified to yield lignin fractions. The lignin fractions obtained were characterized by wet chemistry (carbohydrate analysis) and spectroscopy methods (FT-IR, 13C and 1...

Liu D., Li Y., Zhang G., Zhang P., Wu P., Wang S., Wang X. (2017): Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics. Czech J. Anim. Sci., 62, 89–97. The objective of this study was to investigate the relationship between the protein secondary structure and the protein rumen degradation kinetics ...

Barley grain was sprouted in a still hydroponic growing chamber for 6, 7 and 8 day periods and sampled for chemical analyses, protein fractions, in vitro digestion and metabolisable energy (ME) determination. Productivity measured on the basis of the input-output balance of barley grain and GF yield. Results showed that CP, Ash, EE, NDF, ADF and water soluble carbohydrate (WSC) were increased w...

The carbohydrate composition of an IgA (a, subtype) myeloma protein has been determined. The carbohydrate, present only on the heavy chain, was found to consist of 3 moles of sialic acid, 9 mole of galactose, 5.4 moles of mannose, 0.8 moles of fucose, 8.8 moles of N-acetylglucosamine, and 5 moles of N-acetylgalactosamine per mole of heavy chain. Four major glycopeptide-containing fractions were...

Evidence has been presented for the possible transport function of conjugated roach plasma proteins during molting. Extensive changes in these proteins are evident during the premolt stage. The remainder of the instar appears to be devoted to a gradual return to the intermolt stage. This recovery process is characterized principally by a transformation of less mobile lipoproteins to lipoprotein...

Florisil (activated magnesium silicate) (Saito & Hakomori, 1971 ) or silicic acid (Vance & Sweeley, 1967). The glycolipids recovered from the membranes of unfractionated erythrocytes accounted for 33 %of the galactosamine, 6 % of the glucosamine, but less than 2 of the sialic acid of erythrocyte membranes. When erythrocyte populations from single individuals were fractionated on the basis of de...

The object of this work has been the isolation and identification of the fraction or fractions of pollen which were specifically active in clinical cases of hay fever. The fractions giving an immediate urticarial type of cutaneous reaction have been studied. No attempt was made to identify inactive material. The importance of a close relationship between chemical work and biological tests was e...

Mycelial and yeast-phase culture filtrates prepared from three strains of Paracoccidioides brasiliensis exhibited equal reactivity in sensitized guinea pigs. Ethyl alcohol-precipitated fractions obtained from the culture filtrates also showed no difference in reactivity between mycelial and yeast phase when tested in sensitized guinea pigs. Chemical analyses of the ethyl alcohol-precipitated fr...

An extract from red kidney beans (Phaseolus vulgaris) was fractionated with respect to RNA synthesis-stimulating activity. The activity of the fractions was tested in chicken-spleen lymphocytes cultured in serum-free medium and in plasmolyzed E. coli cells. Lymphocyte-stimulating activity was found in a fraction called F III. This fraction contained protein and orcinol-positive carbohydrate. Th...

1. Although glycoproteins with less than 1% of sialic acid (fibrinogen, lipoproteins, gamma-globulins) interact electrostatically with chondromucoprotein to form insoluble complexes, interaction with glycoproteins containing larger amounts of sialic acid (orosomucoid, urine glycoprotein, seromucoid, fraction VI) was electrostatically impossible. Reasons for this are discussed. 2. The latter gly...