نتایج جستجو برای: caprine spermatozoa

تعداد نتایج: 15991  

2014
Ayumi HASEGAWA Keiji MOCHIDA Toshiko TOMISHIMA Kimiko INOUE Atsuo OGURA

Successful in vitro fertilization (IVF) in mice has been achieved using spermatozoa at concentrations specifically optimized for the experimental conditions, such as species and source of spermatozoa. Although IVF in mice is mostly performed using about 80-500 µl drops, it is expected that the number of spermatozoa used for insemination can be reduced by decreasing the size of the IVF drops. Th...

Journal: :Reproductive biomedicine online 2008
J G Franco R L R Baruffi A L Mauri C G Petersen J B A Oliveira L Vagnini

The aim of this study was to determine the extent of DNA fragmentation and the presence of denatured single-stranded or normal double-stranded DNA in spermatozoa with large nuclear vacuoles (LNV) selected by high magnification. Fresh semen samples from 30 patients were prepared by discontinuous isolate concentration gradient. Spermatozoa with normal nucleus (NN) and LNV were selected at x8400 m...

eliyasi, Mahvash , Jazayeri, Ali , Momtaz, Hassan , Soleimani, Reza ,

Background and Aims: The aim of this study was to determine the virulence and antibiotic resistance properties of Streptococcus agalactiae (S. agalactiae) and Streptococcus uberis (S. uberis) isolated from the ruminant’s sub-clinical mastitic milk in Iran. Materials and Methods: Positive samples in the CMT test were cultured and S. agalactiae and S. uberis have been identified using bi...

2017
Huafang Hao Shengli Chen Yuanguo Li Heting Sun Ping Zhao Yingna Jian Yuwei Gao Changjiang Wu Yongsheng Liu Yuefeng Chu

Mycoplasma capricolum subsp. capripneumoniae is an important pathogen of goats that causes contagious caprine pleuropneumonia. Here, we report the complete genome sequence of M. capricolum subsp. capripneumoniae strain zly1309F, isolated from a Tibetan antelope (Pantholops hodgsonii) in China.

2016
Suhee Kim Sarah Hooper Cansu Agca Yuksel Agca

BACKGROUND Successful cryopreservation of rat spermatozoa from various strains still remains a challenge. The objective of this study was to determine if combinations of OptiPrep™ (iodixanol) and adenosine 5'-triphosphate (ATP) can improve rat sperm function during the cryopreservation procedure. METHODS Epididymal rat spermatozoa were frozen under different OptiPrep™ concentrations (0, 1, 2,...

Journal: :Hiroshima journal of medical sciences 1995
M Kumagai K Ueda Y Shinkoh M Kinutani K Ohama

The acrosome reaction by spermatozoa is an indispensable prerequisite for fertilization, and zonae pellucidae of human oocytes induce this reaction in the human spermatozoa attached to them. Human spermatozoa can attach to the zona pellucida of hamster oocytes when the oocytes have been treated with trypsin. We examined whether or not trypsin-treated hamster zona pellucida induces the acrosome ...

Journal: :Molecular human reproduction 1999
E K Steele N McClure R J Maxwell S E Lewis

Testicular and epididymal spermatozoa are used routinely for intracytoplasmic sperm injection (ICSI) to treat men with obstructive azoospermia. Little is known of the effects of obstruction and stasis on the DNA of these spermatozoa, particularly in the epididymis where spermatozoa have been retained for long periods. Surgical epididymal aspiration for ICSI could provide spermatozoa that are se...

Journal: :Toxicology and applied pharmacology 2008
Jeannett A Izquierdo-Vega Manuel Sánchez-Gutiérrez Luz María Del Razo

Fluorosis, caused by drinking water contamination with inorganic fluoride, is a public health problem in many areas around the world. The aim of the study was to evaluate the effect of environmentally relevant doses of fluoride on in vitro fertilization (IVF) capacity of spermatozoa, and its relationship to spermatozoa mitochondrial transmembrane potential (DeltaPsi(m)). Male Wistar rats were a...

Journal: :Environmental Health Perspectives 1996
J Lähdetie M Ajosenpää-Saari J Mykkänen

We have studied human spermatozoa from 24 normal, healthy unexposed men, 18 of whom were semen donors at the Sperm Bank in Turku, using multicolor fluorescence in situ hybridization with two chromosome-specific probes. The possible age-related increase in aneuploidy frequencies was assessed. Ten thousand spermatozoa were scored per individual for the presence of hyperploid, i.e., disomic and di...

Journal: :Journal of reproduction and fertility 1979
M R Bakst T J Sexton

The fertilizing capacity, motility and ultrastructure of fowl and turkey spermatozoa were examined at various stages of the freezing process. For both species, fertility and motility were depressed after equilibration with dimethyl-sulphoxide at 5 degrees C. After freezing, motility was maintained at 55% for fowl spermatozoa and 40% for turkey spermatozoa; however, fertility was 55% for the fow...

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