CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast directly from clinical specimens, including stool, urine, respiratory, vaginal, orop...

BACKGROUND Opportunistic infections due to Candida species occur frequently in cancer patients because of their inherent immunosuppression. The aim of the present study was to investigate the epidemiology of yeast species from the oral cavity of patients during treatment for oncological and haematological malignancies. METHODS MALDI-TOF was performed to identify yeasts isolated from the oral ...

a radio assay employing 14c-uridine to measure the intracellular killing of candida albicans by phagocytes is presented. the principle of this new method is that after killing of candida albicans by phagocytes rna synthesis stops and therefore uridine uptake is diminished whilst viable candida in cultures would take up uridine and as a result, high cpm counts can be obtained within 135 min. of ...

BACKGROUND Surveillance cultures may be helpful in identifying patients at increased risk of developing invasive candidiasis. However, only scant information exists on the effect of Candida colonization on serum levels of diagnostic biomarkers. This prospective surveillance study determined the extent of Candida colonization among pediatric cancer patients and its possible impact on serum level...

Infection of the oral cavity and dentures by Candida species are frequent in denture wearers. C. albicans is the most common pathogen; however, other emerging Candida species are also responsible for this condition. Few data are available about the occurrence of Candida species in the oral cavities of denture-wearing immigrants to Italy. In this study, we compare the Candida species found in th...

BACKGROUND Fluorescent dye Rhodamine 6G (R6G) is a substrate of multidrug resistance pumps and its accumulation is reduced in some azole-resistant Candida isolates with the upregulation of multidrug efflux transporter genes. Despite reports on species-specific differences in azole susceptibility in various Candida species, only a few studies have been reported on the R6G accumulation among clin...

OBJECTIVE The adherence of Candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. The study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida. METHODS In the former, adsorption to hexadecane was used to measure the hydrophobic interaction of the candida cells. In the later, glass beads c...

objective(s) shark liver oil (slo) is an immunomodulator. macrophages play a key role in host defense against pathogens like fungi. candida albicans have mechanisms to escape immune system. we determined the effect of killed-candida on the in vitro viability of macrophages and the effect of slo on augmentation of this potency. materials and methods peritoneal macrophages were separated and cult...

OBJECTIVES (1) To identify and characterize the Candida species isolates from lower respiratory tract infection. (2) to determine the rate of isolation of Candida species from sputum samples. METHODS This study was carried out in the Department of Microbiology, Manipal Teaching Hospital, Pokhara, Nepal from June 2002 to January 2003. A total of 462 sputum samples were collected from patients ...

conclusions in this study, snpcr was specific and more sensitive than the chromogenic medium chromagar™ candida for the detection of candida spp. insofar as it failed to identify only a few isolates. materials and methods we used snpcr with universal and species-specific primers to detect candida species in the culture of clinical isolates. by using universal primers, we carried out the amplifi...