The best medium for callus proliferation from hypocotyl and cotyledon explants of aseptically grown seedlings of two Egyptian landraces of rare wild plants, Ecballium elaterium and Cucumis prophetarum was found to be Murashige and Skoog (MS) medium supplemented with 0.1-0.5 mg/l NAA in combination with BA at 2 mg/l. On the other hand, MS medium supplemented with 2 mg/l BA, 60 mg/l adenine sulfa...

In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...

Somatic embryogenesis requires auxin and establishment of the shoot apical meristem (SAM). WUSCHEL (WUS) is critical for stem cell fate determination in the SAM of higher plants. However, regulation of WUS expression by auxin during somatic embryogenesis is poorly understood. Here, we show that expression of several regulatory genes important in zygotic embryogenesis were up-regulated during so...

Effects of different concentrations of IAA (0, 0.3, 0.6 mg/l) and 2ip (0, 0.3, 0.6, 0.9, 1.2 mg/l) and their combinations on callus induction and shoot regeneration of hypocotyl and its thin cell layer (TCL) explants in tomato was studied. Explants were prepared from hypocotyls of seedlings in the aseptic condition. Hypocotyl segments were more efficient than TCL explants for callus induction a...

Syngonium podophyllum Schott. cv. ‘White Butterfly’ is recognized as a valuable ornamental plant, and today it also an important plant species of medicinal interest due to its high contents phenolic compounds. The purpose this article review the main scientific publications from our laboratory with regard new achievements dealing or topics interest, such callus formation further de novo shoot r...

-Callus derived from different plant parts of various strains of Cannabi8 8ativa L. was established and analyzed for growth on different media and for can­ nabinoid content by thin-layer (tic) and gas-liquid chromatography (glc). Plant organs which included bracts and anther-calyx complexes of drug (152) and fiber (150) types and maturing leaves of Mexican (drug) and Turkish (fiber) strains wer...

Lagerstroemia indica is an important woody ornamental plant worldwide. However, the application of many technologies, such as transgenic breeding and genome editing, has been severely hampered due to lack efficient calli induction regeneration technology. Here, we discussed a reliable protocol using whole-leaf explants. This protocol’s effectiveness for systems in crape myrtle were up 70.33% 44...