BACKGROUND Tea is an economic important crop with high medicinal value due to rich polyphenols content. In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. OBJECTIVE Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. MATERIALS AND METHODS Standardization of callus inducti...

The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...

Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and a homologous single-copy transgene locus 2 (Lo2), we have studied the stability of the methylation patterns and trans-silencing interactions in cell culture and regenerated tobacco (Nicotiana tabacum) plants. The posttranscriptionally silenced (PTGS) epiallele of t...

Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps of DNA methylation in several regenerated rice lines. We found that all tested regenerated plants had significant losses of methylation compared to non-regenerated plants. Loss of methylatio...

BACKGROUND AND AIMS Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. METHODS Callus development was examined from cultured leaf explants of the high...

optimization of regeneration methods in cotton is necessary to improve and successful production of transgenic cotton cultivars by tissue culture technique. this study was conducted to determine the genotypic effect on callus induction in cotton using 3 genotypes (coker349, 4-s-4 and c1211) and coker 312 selected and a study was carried out in cotton research institute of gorgan using a factori...

The accumulation of kaempferol was evaluated in undifferentiated callus of Tylophora indica through TLC, HPTLC analysis with standard reference compound. Kaempferol is a strong antioxidant and help to prevent oxidative damage to our cells, lipids and DNA. In the present investigation, we have enhanced the kaempferol content in Tylophora indica tissue culture by using precursors like sallicyclic...

Radopholus similis is one of the most damaging nematodes in bananas. Chemical control is currently the most-used method, but nematode control through genetic improvement is widely encouraged. The objective of this study was to establish an aseptic culture system for R. similis and determine whether R. similis can infect and reproduce on in vitro banana plantlets and in vitro Arabidopsis thalian...

Salvia milthiorrhiza root (Danshen) is one of the oldest and most traditional drug of chinese origin, mainly used in the treatment of cardiovascular and cerebrovascular diseases. the tanshiniones (diterpenoids) and phenolic acids are the main biological active substances of S. miltiorrhiza. the aim of this study was to determine the optimal conditions for callus cultures and biosynthesis of the...

We are interested in the cellular physiological events taking place during shoot regeneration in rice (Oryza sativa L. cv. Tainan 5) callus induced by osmotic stress. At first, the sucrose and starch metabolisms in rice callus were studied because carbohydrates are the main energy source in plant tissue culture. The results showed that fresh weight, water content, cellular water, and osmotic po...