Calcitriol, a regulator of calcium homeostasis with antitumor properties, is degraded by the product of the CYP24A1 gene, which is downregulated in human prostate cancer by unknown mechanisms. We found that CYP24A1 expression is inversely correlated with promoter DNA methylation in prostate cancer cell lines. Treatment with the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (DAC) activa...

Calcitonin is a 32-amino-acid peptide hormone that is produced by parafollicular cells of the thyroid gland in response to increased levels of extracellular calcium. In this article we review our previous and current research on the transcriptional regulation of the pig and human calcitonin receptor (CTR) gene. Initially, our experiments on the transcriptional analysis of the pig CTR gene in tr...

Phospholamban is an endogenous inhibitor of sarcoplasmic reticulum calcium ATPase and plays a prime role in cardiac contractility and relaxation. Phospholamban may be a candidate gene responsible for cardiomyopathy. We investigated genome sequence of phospholamban in patients with cardiomyopathy. PCR-based direct sequence was performed for the promoter region and the whole coding region of phos...

S100P gene encodes a calcium-binding protein expressed in different tumor tissues and is functionally implicated in malignant phenotype. Despite consistent relationship to cancer, regulation of S100P gene expression has remained unexplored. Here we determined the transcription start and defined the S100P core promoter. Using a series of the promoter constructs analyzed by dual luciferase report...

many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...

Calcium-stimulated nuclear factor of activated T cells (NFAT) transcription activity at the interleukin-2 promoter is negatively regulated by cyclic AMP (cAMP). This effect of cAMP is mediated, in part, by protein kinase A phosphorylation of NFAT. The mechanism of regulation involves the creation of a phosphorylation-dependent binding site for 14-3-3. Decreased NFAT phosphorylation caused by th...

The transcription factor CREB is involved in mediating many of the long-term effects of activity-dependent plasticity at glutamatergic synapses. Here, we show that activation of NMDA receptors and voltage-sensitive calcium channels leads to CREB-mediated transcription in cortical neurons via a mechanism regulated by CREB-binding protein (CBP). Recruitment of CBP to the promoter is not sufficien...

The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the...