Combination with redox modulators can potentiate the anticancer activity and maximize the selectivity of organometallic complexes with redox-based mechanisms of action. We show that nontoxic doses of l-buthionine sulfoximine increase the selectivity of organo-Os complex FY26 for human ovarian cancer cells versus normal lung fibroblasts to 63-fold. This increase is not due to changes in the mech...

Ferroptosis is a form of cell death caused by iron-dependent lipid peroxidation. Cancer cells increase cystine uptake for the synthesis glutathione (GSH), which used peroxidase 4 to reduce peroxides. Here, we report that deprivation in glioblastoma cells, but not inhibition GSH l-buthionine sulfoximine (BSO), induces ferroptosis. We found decreased protein levels ferritin heavy chain FTH1, wher...

The synthesis of heat shock proteins (HSP) and the development of thermotolerance were studied in Chinese hamster ovary cells in order to determine whether depletion of intracellular glutathione (GSH) inhibited their expression. Cells were exposed to 100 MMdiethylmaleate/50 H.M buthionine sulfoximine which reduced GSH levels by 95% or more during the experimental time course. HSP synthesis was ...