We propose an efficient solution to optimize buffer dumping allowing to reduce significantly the losses caused by buffer overflow. To highlight the proposed architecture we have analyzed its performance compared with different configurations of polling scheme.

Temperature gradient focusing (TGF) is a new and promising equilibrium gradient focusing method which can provide high concentration factors for improved detection limits in combination with high-resolution separation. In this technique, temperature-dependent buffer chemistry is employed to generate a gradient in the analyte electrophoretic velocity. By the application of a convective counter-f...