The parameters that affect the interaction of ligands with a fucose-binding lectin from rat liver have been examined. 125I-Fucosyl-bovine serum albumin (Fuc-BSA) containing 50 residues of fucose/molecule was used as the standard ligand. At low initial concentrations of ligand (10 ng/ml) and lectin (140 ng/ml), the reaction reaches equilibrium at pH 7.8, 23 degrees C, within 40 min. The binding ...

Heats of adsorption of BSA onto polystyrene and polycarbonate were determined microcalorimetrically. The polymers were coated onto 50 nm alumina particles which were immersed in buffer for the measurements. Experimental variations to isolate the various component reactions of the integral heats included adsorption of native and denatured BSA from solution, adsorption onto substrates precoated w...

AIM The aim of this study is to test the potency of bovine serum albumin (BSA) conjugated ampicillin (AMP) and enrofloxacin (ENR) antigens in eliciting an immune response in rats using indirect competitive enzyme-linked immunosorbent assay (icELISA). MATERIALS AND METHODS AMP and ENR antibiotics were conjugated with BSA by carbodiimide reaction using 1-ethyl-3-(3-dimethylaminopropyl) carbodii...

Amadori-modified glycated albumin stimulates extracellular matrix and transforming growth factor-beta (TGF-beta) expression in cultured mesangial cells. Smad proteins transduce the TGF-beta-mediated signal, and Smad-binding CAGA sequences are present in the plasminogen activator inhibitor-1 (PAI-1) promoter. This study examined whether glycated albumin induces PAI-1 transcription in human mesan...

Ginsenosides are triterpenoids that are found in P. ginseng; they have numerous important structural, functional and pharmacological properties. In this work, a desolvation method was used to entrap ginsenoside CK within bovine serum albumin (BSA) to form BSA–CK nanoparticles (NPs), which enhance its aqueous solubility and stability. Following purification, the BSA–CK NPs were characterized by ...

Proteins that have been modified by long-term exposure to glucose accumulate advanced glycosylation end products (AGE) as a function of protein age. In these studies, we have characterized the interaction of AGE-protein with mouse peritoneal macrophages, using AGE-modified bovine serum albumin (AGE-BSA, prepared by incubation with glucose) as a probe. AGE-BSA was specifically bound to cells at ...

To explore the specificity determinants involved in lectin-sugar interactions, we have prepared 6 neoglycoproteins, each containing a single ovalbumin-derived oligosaccharide unit linked to bovine serum albumin (BSA), and compared their concanavalin A (Con A) binding and their in vivo circulatory clearance rate and tissue specificity in the rat. The neoglycoproteins were prepared using the glyc...

An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the...