Interferon regulatory factor 1-deficient (IRF-1(-/-)) mice infected with virulent Brucella abortus 2308 at 5 x 10(5) CFU developed acute hepatitis similar to many natural hosts but, unlike natural hosts, IRF-1(-/-) mice were unable to resolve infection and died. In contrast, IRF-1(-/-) mice survived when infected at 5 x 10(5) CFU with several attenuated Brucella strains (S19, RB51, cbp, and cyd...

A method for the in vitro study of intracellular brucella has been described. Exudative leukocytes containing intracellular brucella have been maintained in vitro in a synthetic tissue culture medium or in human or animal serum. Intracellular brucella are protected in vitro against the lethal action of therapeutic agents or the bactericidal action of serum. This protection of intracellular bruc...

The importance of blood transfusion in stimulating the production of most leuko-agglutinins is established beyond all doubt. Among 60 leuko-agglutinating sera studied by Dausset, 90 per cent were from patients who had received multiple (average of 22) transfusions. Brittingham, Andre et al. and Payne also noted a high degree of correlation. Other than leuko-agglutinins, various types of homolog...

1. Protective substances and agglutinins were not demonstrated in the blood of patients with pneumonia untreated with serum before the fall in the temperature by crisis or lysis. 2. Protective substances and agglutinins were frequently demonstrated in serum treated patients during the course of the disease. 3. Protective substances and agglutinins were usually present at the time of the tempera...

We read with interest the paper “Ribosomal RNA Sequence Analysis of Brucella Infection Misidentified as Ochrobactrum anthropi Infection” by Horvat and colleagues (1). The paper reported that a Brucella isolate was misidentified as Ochrobactrum anthropi. The 16S rRNA results indicated that the isolates were Brucella species, with 100% agreement to rRNA sequences of known brucellae and low homolo...

BACKGROUND To unravel the strategy by which Brucella abortus establishes chronic infections, we explored its early interaction with innate immunity. METHODOLOGY/PRINCIPAL FINDINGS Brucella did not induce proinflammatory responses as demonstrated by the absence of leukocyte recruitment, humoral or cellular blood changes in mice. Brucella hampered neutrophil (PMN) function and PMN depletion did...

T HE discovery of so-called ‘ ‘incomplete’ ‘ or ‘ ‘blocking’ ‘ antibodies and of thermostable agglutinins reacting in serum or albumin but not in saline has stimulated discussion and speculation on their relation to each other and to the previously known Rh agglutinins which react in saline solution, and more recently on their relation to the various forms of fetal erythroblastosis. The problem...

Owing to unavoidable circumstances only a limited amount of time was available for the work on agglutination. The foregoing report is therefore preliminary only and the following conclusions are provisionally offered: I. Pneumococci by reason of their agglutinating properties exhibit a tendency to separate into numerous groups similar to streptococci. II. Pneumococcus mucosus forms a distinct a...

Lipopolysaccharides (LPS) were extracted from rough strains of Brucella abortus and Brucella melitensis and from strains of the naturally occurring rough species Brucella ovis and Brucella canis. Brucella rough lipopolysaccharides (R-LPS) were readily distinguished from Brucella smooth lipopolysaccharides (S-LPS) and enterobacterial R-LPS, by their chemical, physical, and serological characteri...

A previous study (15) has shown tha t transplantable mouse tumors differ in their capacity to grow progressively in rats pretreated with lyophilized mouse tumor tissue. An inverse correlation was found between the degree of strain specificity of tumors in mice and their transplantability to Pretreated rats. The present study deals with the correlation found between the transplantability of mous...