We have used equilibrium dialysis and other techniques to examine the actinomycin D binding sites on unsubstituted and halodeoxyuridine-substituted DNAs. Some of the actinomycin D binding sites present on halodeoxyuridine-substituted DNAs have an enhanced affinity for actinomycin D compared to the standard binding sites present on unsubstituted DNA. Kinetic studies indicate that actinomycin D d...

Background: In the previous decade, various benefits and biological activities of royal jelly, applied in alternative modern medicine, cosmetics, have been reported. However, effects jelly extract (RJ) on keratinocytes not fully elucidated. Objective: The primary objectives this study were to reveal RJ explore underlying mechanism. Methods: HaCaT cells, an immortal human epidermis-derived kerat...

Epithelial cell proliferation was studied in the normal colonic mucosa of 5-week-old Sprague-Dawley rats, comparing [3H]thymidine incorporation (group 1) with two newer proliferation markers, bromodeoxyuridine (group 2) and proliferating cell nuclear antigen (group 3). Microautoradiography (group 1) or immunoperoxidase assays (groups 2 and 3) were carried out. Cells were counted for positive re...

The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of al...

OBJECTIVE Src homology 2-containing phosphotyrosine phosphatase 2 (SHP2) is ubiquitously expressed and believed to function as part of a positive signaling pathway mediating growth factor-induced protein tyrosine phosphorylation. Proliferation of aortic vascular smooth muscle cells (SMCs) is an important contributor to atherosclerosis. We examined the effect of SHP2 expression on SMC proliferat...