نتایج جستجو برای: box pcr
تعداد نتایج: 259656 فیلتر نتایج به سال:
To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially characterized by BOX-PCR genomic DNA fingerprinting, and 60 distinct genotypes w...
To analyze national prevalence, genomovar distribution, and epidemiology of the Burkholderia cepacia complex in Italy, 225 putative B. cepacia complex isolates were obtained from 225 cystic fibrosis (CF) patients attending 18 CF centers. The genomovar status of these isolates was determined by a polyphasic approach, which included whole-cell protein electrophoresis and recA restriction fragment...
Considering the economic importance of chickpea (C. arietinum L.) seeds, it is important to understand the mechanisms underlying seed development for which a cDNA library was constructed from 6 day old chickpea embryos. A total of 8,186 ESTs were obtained from which 4,048 high quality ESTs were assembled into 1,480 unigenes that majorly encoded genes involved in various metabolic and regulatory...
BACKGROUND The present analysis focuses on phenotypic and genotypic characterizations of efflux-mediated erythromycin resistance in Streptococcus pneumoniae due to an increase in macrolide resistance in S. pneumoniae worldwide. METHODS We investigated the prevalence of efflux-mediated erythromycin resistance and its relevant genetic elements from 186 specimens of S. pneumonia isolated from cl...
We solve principle component regression (PCR) by providing an efficient algorithm to project any vector onto the subspace formed by the top principle components of a matrix. Our algorithm does not require any explicit construction of the top principle components, and therefore is suitable for large-scale PCR instances. Specifically, to project onto the subspace formed by principle components wi...
A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolate...
tan spot disease of wheat with the causal agent of pyrenophora tritici-repentis is a very serious disease throughout the world causing severe yield losses. out of 163 isolates of the pathogen, collected from golestan and mazandaran provinces, 58 were selected for a study of population genetic structure. selecting the real representatives from the whole population was carried out according to t...
BACKGROUND AND PURPOSE Twenty lactobacilli isolated from vaginal samples of healthy volunteers were characterized according to polyphasic taxonomy. A broad spectrum of activity and protective properties of these vaginal isolates has been reported. METHODS Phenotypic and genotypic methods such as random amplified polymorphic DNA (RAPD), species-specific and BOX polymerase chain reaction (PCR),...
microRNA-422a (miR-422a) is downregulated in many hematopoietic tumors and solid including hepatocellular carcinoma. We previously demonstrated that hepatitis B virus X protein (HBx) expression of miR-422a HCC cell line HepG2 vitro. However, we explore the mechanisms underlying this action. Forkhead box proteins (FOX) G1/Q1/E1 are known to negatively regulate expression, prompted us determine w...
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