Specific monoclonal antibodies (MAbs) to mefloquine conjugated to bovine serum albumin (mefloquine-BSA) were produced by hybridoma technology. The mefloquine-BSA was synthesized by converting mefloquine into hemisuccinate followed by convalently linked to bovine serum albumin (BSA) and coupling with N,N' disuccinimidyl carbonate (DSC). The conjugate was purified by Sephadex G-75 gel filtration ...

Endomorphins (EMs) have a very important bridge-function in cardiovascular, endocrinological, and neurological systems. This study is to investigate the effects of EMs on the synthesis and secretion of vasoactive substances induced by advanced glycation end products in primary cultured human umbilical vein endothelial cells (HUVECs). Firstly, HUVECs were stimulated with AGEs-bovine serum albumi...

In this study, we report a new method for cholesterol incorporation in mitochondria. This method is based on the coupling of cholesterol to bovine serum albumin (BSA) to form a structure with a spherical shape (designated C-BSA-C). The results show that C-BSA-C induces a specific cholesterol incorporation in mitochondria without interference from BSA. The cholesterol increase is complete in 1 m...

Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin (BSA) was investigated by fluorescence, UV-vis absorbance and circular dichroism (CD). The results showed that the quenching mechanism in the drug-BSA binary systems was a...

This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BSA fluorescence was quenched by eupatorin via a static quenching mechanism. The...

Ehrenwörtliche Versicherung Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet. I Abbreviations µM micromolar APS ammoniumperoxodisulfate BSA bovine serum albumin cDNA copy DNA cpm counts per minute C-terminal carboxyterminal dATP desoxyadenosintriphosphate dCTP desoxycytosintriphosphate DEPC diethylpyrocarbonate DNA desoxyribonucleicacid dNTP desoxynucleosidtriphosphate DTT...

Abstract Owing to the hypoxia status of tumor, reactive oxygen species (ROS) production during photodynamic therapy (PDT) tumor is less efficient. Herein, a facile method which involves synthesis Mg–Mn–Al layered double hydroxides (LDH) clay with MoS 2 doping in surface and anionic layer space LDH was presented, integrate photo-thermal effect imaging catalytic functions LDH. The designed LDH-Mo...

Flavonoid-containing food supplements are widely used as antioxidants, and the continuous use of these with other drugs can lead to clinically significant interactions between drugs. The medications in systemic circulation mainly transported by serum albumin, a major transport protein. This study evaluated rutin (RUT), naringenin (NAR), sinapic acid (SIN) most abundant protein, bovine albumin (...

A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer s...

Abstract Vivianite crystallization is a promising route for phosphorus (P) recovery from P-rich wastewater. However, organic matter (OM) in wastewater may influence vivianite formation. In this study, the effects of four representative OMs, glucose, bovine serum albumin (BSA), humic acid (HA) and sodium alginate (SA), on P by were investigated. The results showed that efficiency was inhibited H...