Previously it had been shown that native lysozyme has three discontinuous antigenic sites (comprising spatially adjacent residues that may be distant in sequence) that were mimicked by surface-simulation synthetic peptides that had the capacity to bind the bulk (97-99%) of the antibody response against native lysozyme. In the present work these three surface-simulation synthetic peptides were c...

It has been suggested by Wiener and Hurst (1947) and Wiener, Hurst, and Sonn-Gordon (1947) that mixtures of bovine albumin and human plasma are more effective as diluents for the detection of incomplete antibodies than the widely employed 20% bovine albumin introduced by Diamond and Abelson (1945) or the human plasma alone used by Wiener (1945). This has been confirmed by other workers, and Mol...

The interaction of naringin with bovine serum albumin has been performed using fluorescence, circular dichroism and fourier transform infrared spectroscopy in 20 mM phosphate buffer of pH 7.0 as well as molecular docking studies. The changes in enthalpy (ΔH°) and entropy (ΔS°) of the interaction were found to be +18.73 kJ/mol and +143.64 J mol K respectively, indicating that the interaction of ...

Bovine serum albumin is commonly used in many biochemical applications due to its stability, lack of interference within biochemical reactions and low production cost. Here, we model the interaction of bovine serum albumin inside a carbon nanotube. The carbon nanotube is chosen as an example to demonstrate its potential use in targeted drug and protein delivery and as an enzyme immobilizing mat...

Hyaluronan (HA) hydrolysis catalysed by hyaluronidase (HAase) is strongly inhibited when performed at low HAase over HA concentration ratio and under low ionic strength conditions. The reason is the ability of long HA chains to form electrostatic and non-catalytic complexes with HAase. For a given HA concentration, low HAase concentrations lead to very low hydrolysis rates because all the HAase...

In this communication we describe serum-free culture conditions for the serial propagation of the C6 glioma cell line. The growth rate, saturation density, and morphology of these cells are equivalent to those of their serum-grown counterparts when cultured in a 3:1 mixture of Dulbecco's modified Eagle's medium and Ham's medium F-12 supplemented with trace elements, insulin, transferrin, fibrob...

To evaluate the re lat ionship be tween tumor burden and c i rculat ing immune complexes (IC) in ch i ldren with neurob lastoma (NBL), we studied sera col lected at intervals from pat ients with d isseminated (Stage III or IV) NBL. Sera from 10 of 12 pat ients conta ined IC by the Raji cell assay at some time dur ing the first 9 to 11 months of the study. H igher IC levels were observed in sera...