conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...

Leukemia was induced in 6 to 8-week-old male and female mice by exposure to fractional doses of X-irradiation. Half the ani mals were given injections immediately after exposure of 40% extracts of sheep spleen according to the procedure of Berenblum et al. (2) or with 10% extracts. The extracts caused a highly signi ficant difference in leukemia incidence in C57BL/6 and BALB/C mico, and no effe...

The purpose of this study was to determine the prevalence of bovine leukemia virus (BLV) in Khorasan Razavi and Khorasan Shomali provinces which are the main provinces located in the northeast of Iran. Total number of 429 blood samples were collected from industrial dairy herds. The samples were categorized based on province, age (2-3, 4-6, and 7-10 years old), calving (≤ 2, 3-5, and > 5) and h...

Degenerate PCR primers which amplify a conserved region of the DNA polymerase genes of the herpesvirus family were used to provide sequence evidence for a new bovine herpesvirus in bovine B-lymphoma cells and peripheral blood mononuclear cells (PBMC). The sequence of the resultant amplicon was found to be distinct from those of known herpesvirus isolates. Alignment of amino acid sequences demon...