For the binding of peptides to wild-type HIV-1 and BIV TAR RNA and to mutants with bulges of various sizes, changes in the DeltaDelta G values of binding were determined from experimental K d values. The corresponding entropies of these bulges are estimated by enumerating all possible RNA bulge conformations on a lattice and then applying the Boltzmann relationship. Independent calculations of ...

HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results f...

We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5'-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expression of a ligand - the BIV Tat peptide.

We present spectroscopic measurements in which utilized are line-intensity ratios in the near-uv to measure electron energies of several hundred eVs, which usually necessitates the use of emission in the soft x-ray region. The main intensity ratio selected is of the BIII transition 1s(2)2s2S(1/2)-1s(2)2p2P3/2 (2066A) and the BIV transition 1s2s3S(1)-1s2p3P2 (2822A). A detailed atomic-kinetics m...

Respiratory disorders are major concern for dairy cattle industry. Viruses and bacteria in combinationwith stress play a key role in triggering acute respiratory infections. The most important viral agents arebovine viral diarrhoea virus (BVDV), bovine herpes virus type 1 (BHV-1), bovine respiratory syncytial virus(BRSV), parainfluenza virus type 3 (PIV-3) and bovine adenovirus (BAV). This cros...

Recently, the discovery of room temperature magnetoelectricity in organic charge transfer complexes has reignited interest in the multiferroic field. The solution processed, large-area and low cost organic semiconductor materials offer new possibilities for the functional all organic multiferroic devices. Here we report the spin polarization of excitons and charge transfer states in organic cha...

The antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. Bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, ...

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

background and aims: genotyping assay has been accepted as a guidance in the therapeutic management of human immunodeficiency virus 1 (hiv-1). but, it is not commonly used in our country due to its high running cost. the aim of this study is evaluate an in-house genotyping resistance test (grt) for hiv-1. methods: hiv-1 rna of 20 samples were extracted from plasma and rt nested- pcr was perform...