Cryptosporidium parvum is a protozoan parasite that causes intestinal infection in a variety of mammals. We have previously described a factor in adult rat or adult bovine intestinal mucosa that protects against C. parvum infection when fed to susceptible infant rats. This factor is absent in intestinal mucosa from bovine calves. In the present study we describe the further characterization of ...

To identify the animal sources for Cryptosporidium and Giardia contamination, we genotyped Cryptosporidium and Giardia spp. in wildlife from Sydney's water catchments using sequence analysis at the 18S rRNA locus for Cryptosporidium and 18S rRNA and glutamate dehydrogenase (gdh) for Giardia. A total of 564 faecal samples from 16 different host species were analysed. Cryptosporidium was identifi...

A total of 256 fecal specimens were randomly collected from farmed poultry in Germany and screened for the presence of Cryptosporidium spp. by PCR and further characterized by direct automated DNA sequencing. Using a nested PCR amplifying approximately 830 bp 18S rDNA fragment, 7.03% (n = 18) of the samples were Cryptosporidium-positive. In detail, Cryptosporidium was detected in 9.3% (8/86) of...

UNLABELLED A number of species of Cryptosporidium are associated with diarrhea worldwide. Little data exists regarding the genotypes and species of Cryptosporidium associated with cases of infections in Brazil. PURPOSE In the present study, we ascertained by molecular methods the species and the genotype of Cryptosporidium sp from a diarrhea outbreak diagnosed in a day care at the Hospital Cl...

Little is known about the epidemiology of Cryptosporidium in Jordan and no genotyping studies have been conducted on Cryptosporidium isolates from humans or animals from Jordan. Genotyping of 44 Cryptosporidium isolates from Jordanian children at the 18S rRNA locus and a unique diagnostic locus identified four Cryptosporidium species; C. parvum (22), C. hominis (20), C. meleagridis (1) and C. c...

The prophylactic and therapeutic effects of the folic acid inhibitor cotrimoxazole (trimethoprim in combination with sulfamethoxazole) was tested in goat kids experimentally infected with Cryptosporidium parvum oocysts. Of the twenty-four 6-day-old kids inoculated with 6 x 10(6) oocysts of C parvum, ten kids were administered cotrimoxazole prophylactically at a dose 20 mg/kg per day of trimetho...

Of 471 specimens examined from foxes, raccoons, muskrats, otters, and beavers living in wetlands adjacent to the Chesapeake Bay, 36 were positive for five types of Cryptosporidium, including the C. canis dog and fox genotypes, Cryptosporidium muskrat genotypes I and II, and Cryptosporidium skunk genotype. Thus, fur-bearing mammals in watersheds excreted host-adapted Cryptosporidium oocysts that...

Giardia lamblia and gastrointestinal illness. Lancet 1985; ii;487-9. 10 Rolston KVI, Fainstein V, Bodey GP. Intestinal cryptosporidiosis treated with eflornithine: a prospective study among patients with AIDS. Journal of Acquired Immunodeficiency Syndromes 1989;2:426-30. 11 Zar F, Geisler PJ, Brown VA. Asymptomatic carriage of cryptosporidium in the stool of a patient with acquired inmunodefici...

Molecular and biological characteristics of a new species of Cryptosporidium from the feces of pigs (Sus scrofa) is described. Oocysts are structurally indistinguishable from those of Cryptosporidium parvum; they are passed fully sporulated, lack sporocysts, and measure 4.9-4.4 microm (mean = 4.6 microm) x 4.0-4.3 microm (mean = 4.2 microm); length to width ratio 1.1 (n = 50). Cryptosporidium s...

Cryptosporidiosis is a clinical disease, usually presenting as a gastro-enteritis-like syndrome, caused by infection with protozoan parasites of the Apicomplexan genus Cryptosporidium. Disease ranges in seriousness from mild to severe and signs and symptoms depend on the site of infection and nutritional and immune status of the host Cryptosporidium in vitro was first achieved in endodermal cel...