Isosorbide dinitrate (ISDN) is an effective drug in treatment of angina pectoris. In this study a new generation of electron capture detector (non-radioactive) with a short, non-polar and wide-bore column was used for analysis of ISDN in human serum. ISDN was extracted from serum by a mixture of ether and ethyl acetate and concentrated at room temperature. The method was linear between 5-50...

Although HPLC-ICPMS has established itself as the analytical technique of choice for elemental speciation analysis, there are some remaining limitations, mainly concerning post-column dispersion effects and the degradation of the ICP stability and ionization efficiency due to organic or saline mobile phases. The application of small-bore columns in conjunction with low dead-volume interfaces pr...

Although the disadvantages of trans fatty acids (TFAs) are widely mentioned, limited data are available on the TFAs contents of Iranian foods, including fast foods. The aim of this study was to quantify the amounts of common fatty acids in several fast foods in Iran, with specific focus on TFAs. The most commonly consumed fast foods in Iran: sausage, calbas, hamburgers and pizzas, were randomly...

Surface porous high-performance liquid chromatography (HPLC) columns were investigated for the separation of kafirins, storage proteins of grain sorghum. Kafirins were successfully separated using C3, C8, and C18 surface porous stationary phases in less than 17 min. Separations using a monolithic C18 stationary phase were also developed and were slightly faster than those achieved on the surfac...

Long-chain acyl-CoA esters (LCACoAs) are activated lipid species that represent key substrates in lipid metabolism. The relationship between lipid metabolism disorders and type 2 diabetes has attracted much attention to this class of metabolites. This paper presents a highly sensitive and robust on-line LC/MS(2) procedure for quantitative determination of LCACoAs from rat liver. A fast SPE meth...

A rapid, accurate RP Levocetirizine and phenylephrine separation was achieved on column Luna 5u C18 (20mm X 4mm) using mobile phase of methanol: Pot. Dihydrogen Phosphate Buffer (pH 6.0), (70:30) .The UV detection was performed at 251nm with flow rate of 1.0 mL Phenylephrine hydrochloride was found to be 8.42 min and 2.70 min with mean recovery 100.64 & 100.40% respectively. The proposed method...

A rapid method for the determination of chondroitin sulfate in raw materials was developed. The samples were finely powdered, dissolved in water, and injected directly into the liquid chromatograph. The method used a C18 column, a wavelength of 195 nm, and a mobile phase containing octane sulfonic acid. The method gave results that were slightly different from those generated by atitrimetric me...