Background: Protection of hematopoietic system has become a primary goal in the development of novel medical countermeasures against ionization radiation and radiotherapy. This study was to explore the role of rapamycin in normal tissues against radiation. Materials and Methods: Mice were pretreated with rapamycin by i.p. every other day for five times before 5 Gy or 8.5 Gy γ-ray whole bo...

Failure of revascularization is known to be the major reason for the poor outcome of pancreatic islet transplantation. In this study, we analyzed whether pseudoislets composed of islet cells and bone marrow cells can improve vascularization and function of islet transplants. Pancreatic islets isolated from Syrian golden hamsters were dispersed into single cells for the generation of pseudoislet...

Objectives- To assess the ectopic bone formation in canine masseter muscle following the implantation of the natural bovine bone minerals (NBM) loaded with canine mesenchymal stem cells (MSCs).Design- Experimental study.Animals- four mongrel dogs.Procedures- Tripotent MSCs isolated from the canine bone marrow were loaded onto the NBM sponges and allowed to adhere. The cell-loaded scaffolds were...

The evaluation of incidence of bone formation by whole syngeneic bone marrow cell suspension and by bone marrow stromal cell cultured in vitro injection into kidney parenchyma was done. Bone tissue was found in 26 kidneys out of 100 injected with whole bone marrow cells suspension. Cultured stromal bone marrow cells grafted into kidney parenchyma produced ossicles in only 4 out of 101 injected ...

introduction: an investigation of bone, cartilage and fat differentiation potentials of canine bone marrow-derived mesenchymal stem cells. materials and methods: bone marrow aspirates were obtained from 10 canine’s iliac crest under general anesthesia. each sample was separately loaded on lymphodex and centrifuged at 1200 rpm for ten minutes to obtain mononuclear cell fraction. 5×104 cell/cm2 o...

acute myeloid leukemia (aml-m7) is a type of pediatric aml accounting for 3–10% of primary childhood aml and children may present with a broad variety of symptoms including low-grade fever, diarrhea, easy bruising, failure to gain weight and life-threatening conditions. we report a rare case of aml .a 26-month-old boy who presented with weakness and fatigue. he was diagnosed as a case of amlm-7...

background: anti-hla-antibodies are known to affect the allograft survival in transplant recipient patients. objective: the aim of this study was to evaluate the association between anti-hla antibodies and kidney allograft outcomes, particularly in recipients with concur-rent donor bone marrow cell infusion (dbmi). methods: between june 2006 and may 2007, forty living unrelated donor kidney tra...

this investigation aimed to examine the protective function of vitamin e on rat bone marrow mesenchymal stem cells (mscs) treated with cadmium chloride. rat bone marrow mscs were extracted using flashing-out and cultured in dmem containing 10% fbs and 100 u/ml pen/strep. at the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride + vit...