Microbes are a major source of antibiotics, pharmaceuticals, and other bioactive compounds. The production of many specialized microbial metabolites is encoded in biosynthetic gene clusters (BGCs). A challenge associated with natural product discovery is that many BGCs are not expressed under laboratory growth conditions. Here we report a genome-mining approach to discover BGCs with luxR-type q...

A method was developed for the heterologous expression of biosynthetic gene clusters in different Streptomyces strains and for the modification of these clusters by single or multiple gene replacements or gene deletions with unprecedented speed and versatility. Lambda-Red-mediated homologous recombination was used for genetic modification of the gene clusters, and the attachment site and integr...

Soil microbiomes are a rich source of uncharacterized natural product biosynthetic gene clusters. Here we use short conserved biosynthetic gene sequences (natural product sequence tags) amplified from soil microbiomes as phylogenetic markers to correlate genotype to chemotype and target the discovery of novel bioactive pentangular polyphenols from the environment. The heterologous expression of...

We report here the 6,512,057-bp draft genome sequence of Bacillus thuringiensis subsp. aizawai HD133. This strain contains at least 6 cry genes and 13 candidate biosynthetic gene clusters.

We announce the complete genome sequence ofBacillussp. strain SDLI1, isolated from larval gut of the stingless beeScaptotrigona depilis The 4.13-Mb circular chromosome harbors biosynthetic gene clusters for the production of antimicrobial compounds.

Kanamycin is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus1). Although it possesses broad range antibacterial activity, bacteria resistant to this antibiotic readily appear, and therefore its clinical use has been restricted to tuberculosis. Resistant bacteria inactivate kanamycin by modifying its specific aminoor hydroxygroup using acetyltransferase, phosphotransferase an...

Abstract Plants represent a rich repository of taxonomically restricted, yet chemically diverse, secondary metabolites that are synthesised via specific metabolic pathways. Enzyme specificity and biosynthetic gene clustering the bottleneck metabolite evolution. As economically important food crops, grape, strawberry, olive produce many pharmaceutically molecules; however, their pathways remain ...

Terpenes and terpenoids contribute aroma flavor that influence consumer preferences in selecting plant-based products. Computational identification of biosynthetic gene clusters (BGCs) plants can pave the way for future genetic engineering. Using integrative genomic, transcriptomic, metabolic pathway annotation analyses, 35 BGCs were identified tobacco with high confidence. Among identified, 7 ...

Recent advancements in genome sequencing and bioinformatic analysis of fungal genomes have revealed that fungi possess cryptic or silent biosynthetic gene clusters (BGCs). This discovery suggests our understanding the universe secondary metabolomes is limited. In this review, we summarize recent strategies for activating BGCs fungi, identify metabolites, highlight their biological activities. W...

Fusarium graminearum is a causal organism of head blight in cereals and maize. Although few secondary metabolites produced by F. are considered disease virulence factors, many molecular products biosynthetic gene clusters expressed during infection their associated role the unknown. In particular, predicted meroterpenoid cluster historically designated as “C16” likely with pathogenicity. Presen...