The objective of this study was to isolate and identify the chemical structure of a biosurfactant produced by Nocardia farcinica strain BN26 isolated from soil, and evaluate its in vitro antitumor activity on a panel of human cancer cell lines. Strain BN26 was found to produce glycolipid biosurfactant on n-hexadecane as the sole carbon source. The biosurfactant was purified using medium-pressur...

Biosurfactant are surface active compounds produced by microorganisms. These molecules reduce surface tension between aqueous solutions and hydrocarbon mixtures. Hundred hydrocarbon saturated soil samples were collected from thirty different areas in Chennai, TamilNadu, India. Seventy two bacterial strains were isolated and cultured by enriching carbon (Glycerol) source. Each culture medium was...

Biosurfactant-producing microorganisms improve the efficacy of hydrocarbon biodegradation as biosurfactant is essential in making hydrocarbons available for breakdown. The present study reports isolation biosurfactant-producing bacteria that can be used crude oil remediation and to characterize generated during breakdown oil. This also evaluating synergism potentiality simultaneous power genera...

a bacterial strain (designated as alcaligenes sp. ms-103) isolated from oil sample of the aghajari oilfield in the south of iran, was able to produce an effective extracellular lipopolysaccharide biosurfactant (1.2±0.05 g/l) on molasses as a sole carbon source. the highest surface tension reduction to level 20 mn/m was achieved by biosurfactant produced by cells grown on molasses under optimum ...

Different screening methods namely, hemolytic assay (HA), modified drop collapse (MDC), tilted glass slide (TGST), oil spread method (OSM), blue agar plate (BAP), hydrocarbon overlaid agar (HOA) plate, emulsification index (EI), emulsification assay (EA) were assessed for their efficiency to detect biosurfactant producing marine bacteria. Forty-five strains of bacteria, comprising 18 Acinetobac...

Paenibacillus macerans TKU029 can produce exopolysaccharides (EPSs; 3.46 g/L) and a biosurfactant (1.78 g/L) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. The biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mN/m at a concentration of 2.76 g/L and reach an emulsification index of 56 % after a 24-h reaction with machine oil. This biosurf...

The present study focused on the evaluation of a nonspecific synergistic effect of biogenic silver nanoparticles (AgNPs) in combination with biosurfactants against environmental bacteria and fungi. The AgNPs were synthesized in the culture supernatants of the biosurfactant producer Bacillus subtilis grown in brewery effluent, molasses or Luria-Bertani media. Antibacterial activities were tested...

A continuous-flow reactor (CSTR) and a soil slurry-sequencing batch reactor (SS-SBR) were maintained in 8l vessels for 180 days to treat a soil contaminated with diesel fuel (DF). Concentrations of Candida tropicalis, Brevibacterium casei, Flavobacterium aquatile, Pseudomonas aeruginosa, and Pseudomonas fluorescens were determined using fatty acid methyl ester (FAME) analysis. DF removal (biolo...

The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbon-utilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDV...

Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16 S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8 pH in the growth medium. Further the partially purified biosurfactants w...