Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evid...

Biofilm production is a major attribute of Enterococcus faecalis clinical isolates. Although some factors, such as sortases, autolysin, and extracellular DNA (eDNA), have been associated with E. faecalis biofilm production, the mechanisms underlying the contributions of these factors to this process have not been completely elucidated yet. In this study we define important roles for the major E...

The ability to simultaneously measure both biofilm thickness and the mass transfer coefficient of an inert tracer through it provides a powerful method to study biofilm development. In this communication previously published data has been collated to interpret global trends in biofilm structure during the transition towards steady-state. It appears that sudden changes in biofilm structure (dire...

introduction: pseudomonas aeruginosa is a gram-negative bacterium that considered as important opportunistic human pathogen. one of the mechanisms that help bacteria to tolerate survival in adverse conditions and resistance to antibiotics is biofilm formation through quorum sensing (qs) signals and toxin-antitoxin (ta) systems. qs and ta are two systems that have important roles in biofilm form...

Pasteurella multocida is an important multihost animal and zoonotic pathogen that is capable of causing respiratory and multisystemic diseases, bacteremia, and bite wound infections. The glycosaminoglycan capsule of P. multocida is an essential virulence factor that protects the bacterium from host defenses. However, chronic infections (such as swine atrophic rhinitis and the carrier state in b...

in this study, high biocontrol potentially bacillus isolates, against root knot nematode were evaluated from viewpoint of biofilm formation and root colonization in vitro. among 139 bacillus isolates from tomato rhizosphere of several regions of razavi khorasan, 15 strains which show more ability to prevent egg hatch and cause larvae mortality of meloidogyne javanica, were tested in biofilm ass...

Background: Paenibacillus larvae is the etiological agent of American foulbrood (AFB) disease, the most lethal disease in honeybee (Apis mellifera) larvae. Aims: The aim of the present work was to study the antimicrobial effect of cell free supernatant (CFS) of probiotics on an Iranian isolate’s biofilm formation. Methods: A l...

AIMS This study examined the contribution of GltB on biofilm formation and biocontrol efficiency of B. subtilis Bs916. METHODS AND RESULTS The gltB gene was identified through a biofilm phenotype screen and a bioinformatics analysis of serious biofilm formation defects, and then a gltB single knockout mutant was constructed using homologous recombination. This mutant demonstrated severe defic...

conclusions sphingomonas paucimobilis can cause serious infections, especially in immunocompromised patients with determined risk factors such as indwelling catheters and diabetes mellitus, due to the effects of multiple virulence factors, together with biofilm formation. results all the clinical isolates of s. paucimobilis produced biofilms. primary bacteraemia (n = 16) was the most common cli...

Staphylococcus aureus intramammary infections (IMIs) have low cure rates using standard antibiotic treatment and increasing the duration of treatment usually improves therapeutic success. Chronic IMIs are thought to be caused by bacteria presenting a specific virulence phenotype that includes the capacity to produce greater amounts of biofilm. In this study, antibiotic susceptibility and biofil...