The global regulator H-NS of Escherichia coli controls genes related to stress response, biofilm formation and virulence by recognizing curved DNA and by silencing acquired genes. Here, we rewired H-NS to control biofilm formation using protein engineering; H-NS variant K57N was obtained that reduces biofilm formation 10-fold compared with wild-type H-NS (wild-type H-NS increases biofilm format...

Staphylococcus aureus is a commensal skin bacterium and causative agent of infectious diseases. Biofilm formation in S. mechanism that facilitates the emergence resistant strains. This study proposes for regulation biofilm through strain-specific physiological changes induced by plant steroid diosgenin. A comparison diosgenin-induced expression regulatory genes associated with revealed intracel...

Introduction: Biofilm formation in Staphylococcus aureus is a major virulence factor. Both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are common causes of community- and hospital-acquired infections and are associated with biofilm formation. The status of biofilm-forming genes has not been explored in Jordanian nasal carriers of S. ...

33 Staphylococcus lugdunensis is a coagulase-negative Staphylococcus that is a commensal of 34 humans and an opportunistic pathogen. It can cause a spectrum of infections including those that are 35 associated with the ability to form biofilm such as endocarditis or indwelling medical devices. The 36 genome sequences of two strains revealed the presence of orthologues of the ica genes that are ...

Staphylococci are frequently isolated from food processing environments, and it has been speculated whether survival after cleaning and disinfection with benzalkonium chloride (BC)-containing disinfectants is due to biofilm formation, matrix composition, or BC efflux mechanisms. Out of 35 food associated staphylococci, eight produced biofilm in a microtiter plate assay and were identified as Stap...

With the opportunistic pathogen Pseudomonas aeruginosa, quorum sensing based on homoserine lactones was found to influence biofilm formation. Here we discern a mechanism by which quorum sensing controls biofilm formation by screening 5850 transposon mutants of P. aeruginosa PA14 for altered biofilm formation. This screen identified the PA3885 mutant, which had 147-fold more biofilm than the wil...

The role of saliva or serum proteins, such as mucin, fibronectin (FN) and mannan-binding protein, on Candida biofilm formation was investigated. Supplementation of saliva with FN had no significant effect on biofilm formation. In contrast, biofilm formation on either mucin-coated or FN-coated acrylic surfaces was significantly less than that of the control. These results suggest that salivary m...

objectives we investigated the potential relationships between biofilm formation and prevalence of virulence genes (asa1, esp, cyla, and gele/spre), and antimicrobial resistance genes (aac (6’)/aph (2”) in enterococcus faecalis isolated from patients with urinary tract infection. patients and methods in this survey 95 e. faecalis isolates from patients with urinary tract infections staying at s...

Shewanella oneidensis COAG, a hyper-aggregating mutant of MR-1, was isolated from a rifampicin-challenged culture. Compared to the wild-type, COAG exhibited increased biofilm formation on glass carrier material. The role of surface-located proteins in the process of COAG auto-aggregation was confirmed by different proteolytic treatments of the aggregates. All of the tested proteolytic enzymes r...