نتایج جستجو برای: biochemical assay

تعداد نتایج: 349260  

Journal: :Current protocols in protein science 2007
Bradley J S C Olson John Markwell

Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in d...

Journal: :jundishapur journal of natural pharmaceutical products 0
leila etemad pharmaceutical research center, mashhad university of medical sciences, mashhad, ir iran zahra oskouei shirvan departments of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran naser vahdati-mashhadian medical toxicology research center, department of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran seyed adel moallem pharmaceutical research center, medical toxicology research center, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran reza zafari departments of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran hossein hosseinzadeh pharmaceutical research center, department of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran; pharmaceutical research center, department of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, ir iran. tel: +98-5138819042, fax: +98-513882 3251

conclusions results from the present study elucidated that treatment with the aqueous extract of l. citriodora leaves was well tolerated via daily ip injection at doses up to 200 mg/kg for a period of 21 days and did not produce any toxicity. the safety of l. citriodora aqueous extract was also confirmed by cell viability assay. objectives the present study evaluated the acute, subacute, and ce...

Journal: :Sensors 2016
Nunzio Cennamo Francesco Chiavaioli Cosimo Trono Sara Tombelli Ambra Giannetti Francesco Baldini Luigi Zeni

An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self...

Journal: :Cancer research 1992
P G Johnston J C Drake J Trepel C J Allegra

Thymidylate synthase (TS) (EC 2.1.1.45) is an important cellular target for the fluoropyrimidine cytotoxic drugs that are widely used in the treatment of solid tumors. Using the TS 106 monoclonal antibody to human TS, we have compared the immunological quantitation of TS by Western immunoblot and immunofluorescent techniques to the conventional biochemical 5-fluorodeoxyuridine monophosphate bin...

Journal: :Journal of Functional Foods 2022

This study aimed to investigate whether Opuntia spp-extracts protect against diclofenac (DF)-induced hepatotoxicity. Rats were pretreated with extracts, betanin (Bet) and N-acetylcysteine (NAC) followed by a single challenge of diclofenac. Liver tissue was collected for biochemical histological analysis. Primary rat hepatocytes treated (400 µmol/L) without pretreatment extract. Apoptosis measur...

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