A supercritical fluid chromatography-based targeted purification workflow using tandem mass spectrometry and molecular networking was developed to analyze, annotate and isolate secondary metabolites from complex mixture. This approach was applied for targeted isolation of new antiviral diterpene esters from Euphorbia semiperfoliata whole plant extract. The analysis of bioactive fractions reveal...

The objective of this work was to evaluate the biochemical composition of six berry types belonging to Fragaria, Rubus, Vaccinium and Ribes genus. Fruit samples were collected in triplicate (50 fruit each) from 18 different species or cultivars of the mentioned genera, during three years (2008 to 2010). Content of individual sugars, organic acids, flavonols, and phenolic acids were determined b...

OBJECTIVE To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi. METHODS In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers. Blast search sequence similarity was found against the existing non redundant nucleotide sequence database ...

Nature, especially the marine environment, provides the most effective drugs used in human therapy. Among the metazoans, the marine sponges (phylum Porifera), which are sessile filter feeders, produce the most potent and highly selective bioactive secondary metabolites. These animals (or their associated symbiotic microorganisms) synthesize secondary metabolites whose activity and selectivity h...

Using double silent substitution, it is possible to generate L-cone and M-cone isolating electroretinograms (ERGs) on a CRT. A major limitation of the technique is that the depth of modulation of cone classes is limited by the restricted luminance of the phosphors and their spectral overlap. To address this problem we have ported the technique to a four-color LED Ganzfeld stimulus (Diagnosis Co...

OBJECTIVE To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus (A. ochraceus) MP2 fungi. METHODS The anti bacterial activity of marine sponge derived fungi A. ochraceus MP2 was thoroughly investigated against antagonistic human pathogens. The optimum inhibitory concentration of the fungi in the elite solvent was also determined. The promising extracts tha...

Actinomycetes have been extensively exploited as one of the most prolific secondary metabolite-producer sources and continue to be in the focus of interest in the constant search of novel bioactive compounds. The availability of less expensive next generation genome sequencing techniques has not only confirmed the extraordinary richness and broad distribution of silent natural product biosynthe...

Extracellular vesicles (EVs) are important players in the communication between different kinds of cells by delivering their content, consisting types RNA, proteins, bioactive lipids, or signaling nucleotides, into target cells. Several EVs distinguished: (1) exosomes with sizes from 30 to 150 nm originate endosomal pathway and form intracellular multivesicular bodies (MVBs), which fuse plasma ...

In recent years, there has been considerable interest in bioactive peptides derived from food proteins, which might benefit human health. Pitaya and jiotilla are fruits with demonstrated biological functions its stem, peel, pulp. However, no studies have conducted to evaluate the peptides. The aim of this study was determine biofunctional properties (antioxidant, antihypertensive, antidiabetic ...

objective: bioactive glass is often used as a filler material for repair of dental bone defects. in different studies osteogenic potential of this material was proved, but its dentinogenesis property is in doubt. the purpose of this study was to evaluate the histological pulp responses of calcium hydroxide and bioactive glass placed directly on exposed pulp tissues. materials and methods: twent...