BACKGROUND Escherichia coli phytase is an acidic histidine phytase with great specific activity. Pichia pastoris is a powerful system for the heterologous expression of active and soluble proteins which can express recombinant proteins in high cell density fermenter without loss of product yield and efficiently secrete heterologous proteins into the media. Recombinant protein expression is infl...

The effects of offering broilers phosphorus-adequate diets containing 10.0 and 11.8 g/kg lysine, without and with 500 FTU/kg exogenous phytase, on growth performance and nutrient utilisation were determined. Each of the four experimental diets was offered to 6 replicates of 10 birds from 7 to 28 days of age. Effects of treatment on performance, apparent metabolisable energy, apparent ileal dige...

The calcium and phosphorous retention of layers was evaluated at different production levels (45 75 95 % of final production peak) with Hy-Line Brown hybrid layers during the first twelve weeks of the laying cycle. 32 layers evenly distributed over 4 treatments (Trts). The Ca content of the diets was identical across all Trts (32,5 g/kg), while P contents and phytase activities differed. In the...

An experiment was conducted to investigate the effect of phytase, organic acids and their interaction on body weight gain, feed consumption, feed conversion ratio, carcass yield and tibia ash. A total of 680 three-day old Japanese quail chicks (Coturnix coturnix japonica) were assigned to 20 battery brooders, 34 chicks in each. The experimental period lasted 35 days. The treatment groups employ...

Ten 56-d-old, 15-kg barrows were surgically fitted with a postvalvular T-cecum cannula at the ileo-cecal junction to evaluate the effect of microbial phytase on apparent and true ileal AA digestibility and N utilization. A semipurified cornstarch- and soybean meal-based diet was formulated to contain 3.4 Mcal of DE/kg, 17.0% CP, 0.8% Ca, and 0.6% P but had a low phytate-P concentration (0.13%; ...

Complexation of dietary phytate with cations is a major cause of reduced bioavailability of Zn and possibly Cu in pig diets. We conducted 2 studies with 2 treatments in young growing pigs (8 to 40 kg) to estimate potential contributions of phytase to availability and supply of Zn and Cu, respectively. Each treatment comprised 10 pens with 8 pigs each as experimental units. In Exp. 1, 500 phytas...

Three experiments involving 162 pigs were conducted to assess the efficacy of phytase (Natuphos; BASF, Mount Olive, NJ) in low-P, corn-soybean meal-based diets. The phytase was produced by a recombinant Aspergillus niger. The phytase supplement contained 5,000 phytase units (PTU)/g. In Exp. 1 (66 pigs) and 2 (60 pigs), growing-finishing pigs were fed fortified corn-soybean meal diets formulated...

Solid-state fermentation (SSF) can divert food waste from landfills and produce high-value products. This study was aimed to investigate the feasibility of using SSF and optimize the conditions of production of phytase by Aspergillus ficuum from potato waste. Different parameters including pH of the potato waste, inoculum level, moisture content, incubation period, temperature, and supplementar...

Phytases are hydrolytic enzymes that initiate the release of phosphate from phytate In recent years the application of the phytase enzyme have been studied intensively. Phytase enzymes have a wide distribution in plants, microorganisms, and in some animal tissues. Many strategies have been developed for improving phosphate and mineral availability in feed. However in recent years, expression of...

A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range an...