In the healthy hepatocyte, uptake of bile acids across the basolateral membrane and export via the canalicular export pump, are tightly coupled. Impairment of bile formation or excretion results in cholestasis, characterized by accumulation of bile acids in systemic blood and within the hepatocyte. When the concentration of bile acids exceeds the binding capacity of the binding protein located ...

A method is described for the separation of a diarrhoeal stool into solid and liquid phases. The seven patients studied had varying degrees of ileal resection. Faecal bile acids were measured in supernatant and pellet. Freeze-dried pellet of the patients had significantly higher bile acid content than freeze-dried faeces of the eight controls (patients mean = 55.8 mg bile acids/ g freeze-dried ...

The dissociation constants for the carboxyl group of a series of glycine (N-acyl)-conjugated and unconjugated bile acids were determined by potentiometric titration using dimethylsulfoxide-water and methanol-water mixtures of varying proportions. The pKa values in water were calculated by extrapolating the experimental values determined in different mole fractions of the organic solvent mixture...

Bile acids in the plasma, urine, and small intestine of adult male rats with occluded bile ducts have been studied using a method of high specificity for their determination. After bile duct ligation cholic acid rapidly accumulates in the plasma for 8 hr, remains high for a further 8 hr, and subsequently diminishes; bile acids disappear from the small intestine. During the first 12 hr after bil...

A simple, sensitive, and specific liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) method for the determination of bile acids in human bile has been developed. The bile acids were extracted with a C(18) (octadecyl) reversed-phase column and identified and quantified by simultaneous monitoring of their parent and daughter ions, using the multiple reaction monitoring mode. I...

Iso-bile acids, the BP-hydroxy epimers of the normally occurring bile acids, are commonly found in the feces of man and other animals but are not present in the bile. Since isochenodeoxycholic acid and isoursodeoxycholic acid were recently isolated from the feces of patients treated with chenodeoxycholic acid or ursodeoxycholic acid, the intestinal absorption and hepatic metabolism of [24-‘4C]i...

UDCA renders bile acid composition more hydrophilic, shifts the bile-acid/phospholipid ratio towards less toxic effects and, thus, might protect against hepatobiliary injury by hydrophobic bile acids. UDCA is a constituent of human bile in a low concentration of 3% of total bile acids. It is the major bile acid of the bile of black bears and has been used for centuries in traditional Chinese me...

Bile acids are implicated as aetiological factors in many types of gastrointestinal tract cancer including cholangiocarcinoma (CCA). Alterations in bile acid concentrations may affect the pathogenesis of these different types of cancer. Our aim was to determine the bile acid profile in gallbladder bile from patients who underwent liver resection. Thirty-seven patients with cholangiocarcinoma, 5...

Luminal free fatty acids and bile acids may damage the colonic epithelium and stimulate proliferation, which may increase the risk of colon cancer. It has been suggested that only soluble calcium ions (Ca2+) precipitate fatty acids and bile acids, thus reducing their lytic activity. Consequently, precipitation of luminal Ca2+ by dietary phosphate should inhibit these effects. To evaluate the pr...