A D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase (Xfp) from the probiotic Bifidobacterium lactis was purified to homogeneity. The specific activity of the purified enzyme with D-fructose 6-phosphate as a substrate is 4.28 Units per mg of enzyme. K(m) values for D-xylulose 5-phosphate and D-fructose 6-phosphate are 45 and 10 mM, respectively. The native enzyme has a molecular mass...

This study investigates the possible synergistic stimulatory effects of prebiotic chitooligosaccharide and some commercial prebiotics on probiotic growth. Different combination with inulin, fructooligosaccharide, lactulose were prepared their ability to stimulate growth strains was evaluated. Following Chou-Talalay method, index calculated used for assessment. The data showed that most combinat...

Gastrointestinal (GI) adverse effects such as erosion and increased permeability are common during the use of nonsteroidal anti-inflammatory drugs (NSAIDs). Our objective was to assess whether Bifidobacterium animalis ssp. lactis 420 protects against NSAID-induced GI side effects in a rat model. A total of 120 male Wistar rats were allocated into groups designated as control, NSAID, and probiot...

The intestinal overgrowth of Clostridium difficile, often after disturbance of the gut microbiota by antibiotic treatment, leads to C. difficile infection (CDI) which manifestation ranges from mild diarrhea to life-threatening conditions. The increasing CDI incidence, not only in compromised subjects but also in traditionally considered low-risk populations, together with the frequent relapses ...

statement of the problem: dental caries is the most common disease of childhood. using probiotics has recently been introduced to reduce the incidence of dental caries.it consists of live microbial food supplements that beneficially affect the host, and hence are considered an alternative way to eradicate the infections. purpose: the aim of this study is to evaluate the effect of consumption of...

background: the application of natural compounds including green tea extract (gte) in food and pharmaceutical industries is limited. encapsulation in nanoliposomes could be used as a delivery system to protect these compounds during processing and storage. objective: in this study encapsulation of green tea extract in nanoliposomes and evaluation of its antibacterial, antioxidant and prebiotic ...

BACKGROUND There are some evidences for the immunomodulation disorders in the response to intestinal microbiota in inflammatory bowel disease. Yogurt is a fermented milk product made with a starter culture consisting of different probiotics which could be colonized in intestine. However, the role of probiotics in the aetiopathogenesis of ulcerative colitis (UC) has not been clarified. To determ...