achillea millefolium belonging to the astreaceae family is an endangered medicinal plant of jordan and of its neighboring countries. as an alternative to seed propagation, an efficient micropropagation of a. millefolium and its subsequent rooting were developed as an option for its in vitro conservation. a maximum of 5.9 shoots per microshoot were obtained on murashige and skoog agar medium sup...

The magnitude of demand for planting materials in grape, mainly for rootstock genotypes indicates that micropropagation is inevitably necessary for their mass scale propagation. Therefore, the studies on micropropagation of four genetically different grape rootstocks namely Dogridge (Vitis champini), SO4 (V. riparia× V. berlandieri), H-144 (V. vinifera × V. labrusca) and 3309 C (V. riparia × V....

[15N]Aspartate and 5-amino-4-imidazolecarboxamide riboside (AICAriboside) were used to evaluate the contribution of the purine nucleotide cycle to ammonia production in renal tubules isolated from control and chronically acidotic rats. Addition of 1 mM AICAriboside to incubation medium containing 2.5 mM [15N] aspartate significantly stimulated production of 15NH3 and 15N in the 6-amino group of...

In this study appropriate hydrazide compounds, furan-2-carboxylic acid hydrazide (1) and phenylacetic acid hydrazide (2) were converted into 1,4-substituted thiosemicarbazides 4a-e and 5a-e and 4-amino-5-(furan-2-yl or benzyl)-4H-1,2,4-triazole-3-thiols 7 and 10. The 1,4-substituted thiosemicarbazides were then converted into 5-(furan-2-yl or benzyl)-4-(aryl)-4H-1,2,4-triazole-3-thiols 8a-e and...

GAR transformylase, which is responsible for the incorporation of formate carbon into what becomes position 8 of the purine ring, has been completely separated from 5-amino-4-imidazolecarboxamide ribotide transformylase (3). The latter enzyme is responsible for the incorporation of formate carbon into position 2 of the purine ring. The requirement for folic acid compounds has been demonstrated ...

In addition to the bound purine moieties of DNA and RNA, plants and animals contain a variety of other purine derivatives ranging in decreasing structural complexity from free nucleotides to simple substituted bases. Many of these compounds play important roles in metabolism and in its control and integration. Whereas most of the purine derivatives found in animal tissues also occur in plants, ...

Newly synthesized tocainide analogs were tested for their state-dependent affinity and use-dependent behavior on sodium currents (INa) of adult skeletal muscle fibers by means of the Vaseline-gap voltage clamp method. The drugs had the pharmacophore amino group constrained in position alpha [N-(2,6-dimethylphenyl)pyrrolidine-2-carboxamide (To5)] or beta [N-(2,6-dimethylphenyl)pyrrolidine-3-carb...

The intraerythrocytic human malaria parasite, Plasmodium falciparum, requires a source of hypoxanthine for nucleic acid synthesis and energy metabolism. Adenosine has been implicated as a major source for intraerythrocytic hypoxanthine production via deamination and phosphorolysis, utilizing adenosine deaminase and purine nucleoside phosphorylase, respectively. To study the expression and chara...