Upregulation of the MDR1 (multidrug resistance 1) gene is involved in the development of resistance to antifungal agents in clinical isolates of the pathogen Candida albicans. To better understand the molecular mechanisms underlying the phenomenon, the cis-acting regulatory elements present in the MDR1 promoter were characterized using a beta-galactosidase reporter system. In an azole-susceptib...

A method with the combined advantages of soil particle washing, selective inhibitors, and an indicator substrate was developed to isolate saprophytic basidiomycetes from soil. Organic particles were washed from soil and plated on a medium containing lignin, guaiacol, and benomyl, which reduced mold growth and allowed detection of basidiomycetes producing laccase or peroxidase. The 64 soil sampl...

The KlYAP1 and KlPDR1 genes encode two main transcriptional regulators involved in the control of multidrug resistance in Kluyveromyces lactis. Deletion of KlPDR1 or KlYAP1 genes in K. lactis generated strain hypersusceptible to diamide, benomyl, fluconazole and oligomycin. Overexpression of genes KlPDR1 or KlYAP1 from a multicopy plasmid in the Klpdr1Δ mutant strain increased the tolerance of ...

We have examined the effects of the antimicrotubule agent benomyl and several mutations on nuclear and mitochondrial movement in germlings of the filamentous fungus Aspergillus nidulans. While, as previously reported, benomyl inhibited nuclear division and movement, it did not inhibit mitochondrial movement. To test the effects of benomyl more rigorously, we germinated two benomyl super-sensiti...

esca of grapevine is a serious and injurious disease in grape-growing all over the world, for which no effective control exists. the presented research ,in two separate experiments ,thirteen fungicides (benomyl, thiophanate-methyl thiram, mancozeb, fosetyl-al, metalaxyl mancozeb, caboxin thiram, ortiva, topas, penconazole , tebuconazole ,propiconazole, sodium arsenate, agri-fos ) were evaluated...

Fusarium root rot, caused by Fusarium oxysporum schl. f. sp. pini (Hartig) Snyder & Hansen, causes severe losses to some species of conifers even though beds are fumigated with methyl bromide-chloropicrin (3:2) the autumn before spring seeding. Benomyl (methyl-l-(butylcarbamoyl)-2-benzimidazole carbamate) at ll.2 kg a.i./ha or captan (N-trichloromethylthio-4cyclohexene-l,2-dicaboximide) at 7.3 ...