Malaria in Goa, India, has been endemic ever since an outbreak occurred in 1986. Anopheles stephensi Liston has always been suspected as a malaria vector in this area. Due to lack of knowledge on its resting behavior, sufficient adult females could not be collected and incriminated as vectors in the past. In this study mosquito collections were conducted in three endemic urban and suburban area...

Wolbachia is a maternally transmitted symbiotic bacterium of insects that has been proposed as a potential agent for the control of insect-transmitted diseases. One of the major limitations preventing the development of Wolbachia for malaria control has been the inability to establish inherited infections of Wolbachia in anopheline mosquitoes. Here, we report the establishment of a stable Wolba...

Establishment of laboratory colony is essential for mosquito-borne-disease research. Mating behavior of stenogamous Anopheles peditaeniatus and seven eurygamous species (Anopheles argyropus, Anopheles crawfordi, Anopheles nigerrimus, Anopheles nitidus, Anopheles paraliae (=An. lesteri), Anopheles pursati and Anopheles sinensis), were investigated and compared in this study. The self-mating succ...

A causative agent of human malaria, Plasmodium falciparum, is transmitted by Anopheles mosquitoes. The malaria parasite is under intensive attack from the mosquito's innate immune system during its sporogonic development. We have used genetic engineering to create immune-enhanced Anopheles stephensi mosquitoes through blood meal-inducible expression of a transgene encoding the IMD pathway-contr...

BACKGROUND & OBJECTIVES Repellents are commonly used personal protection measures to avoid mosquito bites. In the present study, Advanced Odomos cream (12% N, N-diethyl-benzamide) was tested for its efficacy against mosquitoes in comparison to DEET (N,N-diethyl-3-methyl benzamide). METHODS Bioassays were conducted to assess the repellency of Advanced Odomos and DEET creams against Anopheles s...

Abstract Mosquito vectors in the present universe cause a major problem due to transmission of pathogens with high morbidity. The research aimed explore larvicidal and adulticidal toxicity Cladostepus spongiosus extract its fabricated AgNPs on key mosquito vectors. synthesized were confirmed by UV-Vis spectroscopy, electron microscopy, energy-dispersive X-ray Fourier transform infrared spectrop...

The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and prepa...

Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human malaria vector, Anopheles stephensi. The system consists of six transgenic lines of Anopheles stephensi, each with a single piggyBac-Gal4 element in a uni...

b a ckground : malaria still remains a public health problem in iran. there are different vector control interventions such as insecticide spraying. the present study was carried out to determine the susceptibility status of anopheles stephensi larvae to temephos as a national plan for monitoring and mapping of insecticide resistance m e t hods : eight different localities in two main malarious...

background: anopheles mosquitoes are an important group of arthropods due to their role in transmission of ma­laria. the present study was conducted for determination of susceptibility status of anopheles stephensi to different imagicides collected from malarious area in chabahar city, iran. methods: in the present study seven insecticides including: ddt 4%, lambdacyhalothrin 0.05%, deltamethri...