Two independent experiments were conducted with broilers to determine the effect of threonine and vitamin A on immune system. In first experiment, dietary treatments contained 0.8%, 0.87%, 0.94 % and 1.01 % total threonine. In second experiment, diets were as follows: basal diet without vitamin A; basal diet plus 1500 IU/Kg vitamin A; basal diet plus 6250 IU/Kg vitamin A; basal diet plus 11000 ...

BACKGROUND Dengue virus (DENV) infection can cause severe hemorrhagic disease in humans. Although the pathogenic mechanisms underlying severe DENV disease remain unclear, one of the possible contributing factors is antibody-dependent enhancement (ADE) which occurs when sub-neutralizing antibodies derived from a previous DENV infection enhance viral infection through interaction between virus-an...

BACKGROUND The management of peanut allergy relies on allergen avoidance and epinephrine autoinjector for rescue treatment in patients at risk of anaphylaxis. Biomarkers of severity and threshold of allergic reactions to peanut could significantly improve the care for patients with peanut allergy. OBJECTIVE We sought to assess the utility of the basophil activation test (BAT) to predict the s...

Results 44 peanut allergic children (median age 5 years) reacted to peanut on DBPCPC with clinical symptoms than ranged from oral allergy syndrome to anaphylaxis. 61% of patients reacted to 0.1g of peanut protein. The mean %CD63+ basophil at 10 and 100 ng/ml of PE was independently associated with severity (p=0.012) whilst CD-sens (1/EC50x100) was independently associated with threshold (p=0.03...

Purpose Several studies have shown the relationship between eosinophilia and gastrointestinal allergy in preterm infants; however, its mechanism has not yet been established. Interleukin (IL)-5 was shown to be involved in allergic inflammation by promoting eosinophil differentiation and activation and inducing basophil production. In this study, we examined the changes in eosinophil and basophi...

Methods Monoclonal human IgE antibodies specific for the major birch pollen allergen Bet v 1 and for the major grass pollen allergen Phl p 5 and the corresponding allergens as well as a hybrid protein consisting of the Bet v 1 and Phl p 5 allergens were expressed. Using circular dichroism spectroscopy we compared the fold of the hybrid protein with that of the mixture of the two allergens. Rat ...

Methods Purified native peanut conglutin, containing Ara h2 and Ara h6, was chemically modified by reduction and alkylation (RA), without or with additional glutaraldehyde treatment (RAGA). Serum of 14 peanut allergic patients (DBPCFC-confirmed) was analyzed for peanut-specific antibodies by IgE immunoblot and IgE/IgG1/IgG4 ELISA. Allergen-induced basophil activation was analyzed by direct baso...