INTRODUCTION Oxidative phosphorylation dysfunction of hepatocyte mitochondria is involved in the pathophysiology of organ dysfunction following obstructive jaundice (OJ). However the time period from biliary occlusion to the occurrence of the dysfunction has not been determined decisively. PURPOSE To evaluate the early effects (1 d and 7 d) of OJ on liver mitochondria respiratory function in ...

The measurement of soil carbon dioxide (CO2) respiration is a means to gauge biological soil fertility. Test methods for respiration employed in the laboratory vary somewhat, and to date the equipment and labor required have limited more widespread adoption of such methodologies. A new method to measure soil respiration was tested along with the traditional alkali trap and titration method. The...

To evaluate the physiological functions of β1-, β2-, and β3-adrenoceptors (ARs) in brown adipose tissue, the lipolytic and respiratory effects of various adrenergic agonists and antagonists were studied in rat brown adipocytes. The β-agonists stimulated both lipolysis and respiration (8-10 times above basal levels), with the following order of potency (concentration eliciting 50% of maximum res...

results the mean age in breathing technique with lavender and breathing technique alone were 25.5 ± 4.3 and 26 ± 4.9, respectively .two groups were comparable in this regards (p = 0.6), but the length of active phase in interventional group was 7.85 ± 3.85 hours and in the control group it was 9.88 ± 6.65 hours. the decrease of the length of labor in the active phase was higher in the experimen...

The inhibitory effect of high and low molecular weight native and synthetic rat atrial peptides on oxygen consumption in isolated rat kidney mitochondria and slices was measured. Oxygen consumption by mitochondria was measured in the presence of succinate and/or adenosine diphosphate, furosemide, and low and high molecular weight native and synthetic rat atrial peptides. After the addition of s...

Current commercially available instruments for monitoring mitochondrial respiration are incapable of single cell measurements. Therefore, we developed a three-electrode, Clark-type biosensor suitable for mitochondrial respirometry in single oocytes and embryos. The biosensor was embedded in a PMMA (polymethyl methacrylate) micro-chamber to allow investigation of single oocytes/embryos immersed ...

This study aimed to explore whether angiotensin II (Ang II) inhibits the proliferation of human umbilical vein endothelial cells (HUVECs) by changing mitochondrial energy metabolism, and whether atorvastatin has a protective role via restoration of endothelial function. HUVECs were treated with 1 µM Ang II alone or with 10 µM atorvastatin for 24 h. Proliferation was detected by MTT assay, cell ...

Isotope discrimination is a common feature of biosynthesis in nature, with the result that different classes of carbon compounds frequently display different (13)C/(12)C ratios. The (13)C/(12)C ratio of lipid in potato tuber tissue is considerably lower than that for starch or protein. We have collected respiratory CO(2) from potato discs in successive periods through 24 hr from the time of cut...

Background: Age-related impairment of classical neutrophil functions is well described (Fortin, McDonald et al. 2008). However, experimental evidence for age-related alterations metabolic adaptation towards nutrient deprivation -a feature battlefields- remains elusive. Moreover, differences in may contribute to pathologies such as atherosclerosis, cancer, and autoimmune diseases including rheum...