Gibberellic acid-induced alpha-amylase synthesis in barley (Hordeum vulgare L.) aleurone layers was inhibited by abscisic acid, and the inhibition was partly removed by additional gibberellic acid alone and by ethylene alone. Together additional gibberellic acid and ethylene almost eliminated abscisic acid inhibition of amylase synthesis. Time course studies of these phenomena showed that the e...

Six-day-old seedlings of barley (Hordeum vulgare L., cv. Hemus) were placed in darkness in order to induce senescence-like changes. The symptoms of senescence and the reversibility of the processes were followed by analyses of the content of leaf pigments, the levels of total soluble and Rubisco immunoreactive protein and total proteolytic activity with casein as a substrate. Rubisco specific d...

Kernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18 degrees C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for alpha-amylase components by isoelec...

Initial dark fructose 2,6-bisphosphate levels in 10-day-old barley (Hordeum vulgare L.) leaves increased when the photosynthetic period was lengthened, when the temperature during the prior photosynthetic period was reduced, and following leaf excision. These treatments also increased the leaf sucrose concentration. Conversely, a decrease in dark fructose 2,6,-bisphosphate occurred during exten...

Glycine, a photorespiratory intermediate, enhanced the in vivo reduction of nitrate in barley (Hordeum vulgare L.) leaf slices, when included in the assay medium. Isonicotinyl hydrazide, an inhibitor of glycine oxidation, partially reduced NO(2) (-) production. The enhancement caused by glycine treatment was reversed by isonicotinyl hydrazide when both were present together in the medium. Simil...