Many studies using mammalian cellular and subcellular systems have demonstrated that polycyclic aromatic hydrocarbons, including benzo[a]pyrene (BaP), are metabolically activated by cytochrome P450s (CYPs). In order to evaluate the role of hepatic versus extra-hepatic metabolism of BaP and its pharmacokinetics, we used the hepatic cytochrome P450 reductase null (HRN) mouse model, in which cytoc...

Chemical-DNA adducts provide an integrated measure of exposure, absorption, bioactivation, detoxification, and DNA repair following exposure to a genotoxic agent. Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon (PAH), can be bioactivated by cytochrome P-450s (CYPs) and epoxide hydrolase to genotoxic metabolites which form covalent adducts with DNA. In this study, we utilize...

Para conservar ex situ, promover el uso y la potenciación de germoplasma nativo interés ornamental como T. viereckii subsp. major una especie en estatus riesgo Pr, se planteó presente investigación con objetivo regenerar esta laboratorio cultivo tejidos vegetales (LCTV) del Campo Experimental Saltillo CIRNE-INIFAP considerando estabilidad explante durante subcultivos subsecuentes, determinar ti...

The production of strawberry planting material using the method clonal micropropagation plants is promising, modern and already widely used. This paper presents results assessing breeding potential garden varieties Nelly Kemiya (selection FSBSI NCFSCHVW) in vitro culture. Apexes from growing rosettes strawberries served as starting for initiation. best survival rate explants was noted during in...

BAP is a publicly available infrastructure for performing program verification and analysis tasks on binary (i.e., executable) code. In this paper, we describe BAP as well as lessons learned from previous incarnations of binary analysis platforms. BAP explicitly represents all side effects of instructions in an intermediate language (IL), making syntaxdirected analysis possible. We have used BA...

The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Black Satin’ (Rubus fruticosus L.) and ‘Tayberry’ (Rubus fruticosus x Rubus idaeus). Nodal segments carrying dormant buds were used for in vitro culture establishment. For shoot initiation, MS medium containing 8 g*L phytoagar, 30 g*L sucrose, 1 mg*L ...

Sprouted corm sections of "South Dade" white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3-5 mm comprising the apical meristem with 4-6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg's B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA...

The present research work involves shoot formation, their multiplication and rooting in carnation Dianthus caryophyllous. For shoot formation both apical and nodal meristems were used. MS medium containing BAP alone or in combination with kinetin was tested. Best shoot formation response was obtained after 6 days of inoculation from apical meristem and after 7 days of inoculation from nodal mer...

The biodegradation of benzo[a]pyrene (BaP) by using Polyporus sp. S133, a white-rot fungus isolated from oil-contaminated soil was investigated. Approximately 73% of the initial concentration of BaP was degraded within 30 d of incubation. The isolation and characterization of 3 metabolites by thin layer chromatography, column chromatography, and UV-vis spectrophotometry in combination with gas ...