A technique has been described for the cultivation in vitro of normal mononuclear cells on glass slides in a liquid medium. Under these conditions the monocytes transformed into macrophages which proliferated as in ordinary tissue culture. These cultures of monocytes could be infected with tubercle bacilli. The numbers of stainable tubercle bacilli within the monocytes increased steadily in cul...

In a previous number of this Journal t the writer published an article "Upon the Presence of the Typhoid Bacillus in the Urine," and, as the result of observations upon 38 cases of typhoid fever, drew the following conclusions: (1) Typhoid bacilli were demonstrated in 9 out of the 38 cases (about 25 per cent). (2) The bacilli, when demonstrated, were always present in large numbers, and in prac...

MICE WERE INJECTED INTRAPERITONEALLY WITH ONE OF THE FOLLOWING BACTERIAL PRODUCTS HAVING ENDOTOXIN ACTIVITY: pertussis vaccine, a suspension of heat-killed cells of Klebsiella pneumoniae (type C), or a purified lipopolysaccharide prepared from cultures of Salmonella typhosa. Following treatment with either one of these materials, the animals were infected intravenously with virulent cultures of...

Tubercle bacilli labelled with C(14) were prepared by growth on radioactive substrates such as glycerol, CO(2), and acetate. These organisms were exposed in vitro to leucocytes (mostly polymorphonuclear leucocytes) from peritoneal exudates of guinea pigs. The respiration of the leucocytes and of the bacilli, alone and together, was followed by determining oxygen uptake and C(14)O(2) production....

At varying periods of time following the successful establishment of systemic infections with Mycobacterium leprae or M. lepraemurium in the mouse and the nine-banded armadillo eyes were examined by light microscopy. Inoculation of bacilli was by the intravenous or intraperitoneal route or directly into the hind footpads; eyes were not directly inoculated in this study. During periods of up to ...

Before the development of egg mediums, serum was used extensively as a medium in the cultivation of tubercle bacilli. Eastwood in 1907 reported that horse, bovine, dog, and pig serums supported good growth of stock cultures of human and bovine tubercle bacilli and also served well as isolation mediums. Since the introduction of egg mediums there has been little use of serum as a medium in itsel...

Epidemiological, clinical, and histopathological evidence suggests that black people are more susceptible to tuberculosis than are white people. The cellular basis of this putative susceptibility was investigated in vitro by comparing responses of blood-derived macrophages from black and white donors to experimental infection with virulent tubercle bacilli. Phagocytes from pairs of black and wh...

THE bacteria which may be included together as the Gaertner Group are of considerable imnportance, comprising as they do most of the bacteria fotund in outbreaks of food poisoning and in paratyphoid fever, while they are etiologically concerned with a number of diseases in aninmals both domesticated and wild. The arrangement of bacteria into groups is very convenient and has been extensively pr...

1. There is an extracellular factor which inhibits the growth of tubercle bacilli in immunized rabbits. 2. Extracellular factors localize carbon particles, trypan blue and tubercle bacilli at the site of introduction to a greater extent in the immunized than in the normal animal. 3. This greater fixation is brought about by an increase in the density and extent of the fibrin barrier formed abou...

A previously reported form of microbial persistence whereby large populations of tubercle bacilli can be made to "vanish" uniformly from the tissues of mice has been shown to occur generally throughout each group of animals subjected to the experimental procedure; it does not reflect the eradication of the bacilli in the majority of animals with their persistence and ultimate revival in only a ...