(R)-stereospecific amine transaminases (R-ATAs) are important biocatalysts for the production of (R)-amine compounds in a strict stereospecific manner. An improved R-ATA, ATA-117-Rd11, was successfully engineered for the manufacture of sitagliptin, a widely used therapeutic agent for type-2 diabetes. The effects of the individual mutations, however, have not yet been demonstrated due to the lac...

BACKGROUND Aspirin-exacerbated respiratory disease (AERD) refers to the development of bronchoconstriction in asthmatics following the ingestion of aspirin. Although alterations in eicosanoid metabolites play a role in AERD, other immune or inflammatory mechanisms may be involved. We aimed to identify proteins that were differentially expressed in nasal polyps between patients with AERD and asp...

Renal hypoxia is thought to be an important pathophysiological factor in the progression of chronic kidney disease (CKD) and the associated hypertension. In a previous study among CKD patients, supplementation with 100% oxygen reduced sympathetic nerve activity (SNA) and lowered blood pressure (BP). We aimed to assess the underlying haemodynamic modulation and hypothesized a decreased systemic ...

3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput C...

kuh-e-siah is located in the north of gavkhuni lagoon and east of the varzaneh (se of isfahan) and is a part of urumieh-dokhtar magmatic arc (udma). the volcanic rocks of this area are vesicular basalt and basaltic andesite with late miocene age. the most common textures of these rocks are porphyry, microlitic porphyritic, hyaloporphyric and vesicular. plagioclase, olivine and clinopyroxene are...

Background Thyroid cancer diagnosis has evolved to include computer-aided (CAD) approaches overcome the limitations of human ultrasound feature assessment. This study aimed evaluate diagnostic performance a CAD system in thyroid nodule differentiation using varied settings. Methods Ultrasound images 205 nodules from 198 patients were analysed this retrospective study. AmCAD-UT software was used...

BACKGROUND Benefits of 2-dimensional (2D) angiosome-oriented infrapopliteal revascularization remain controversial. The aim of this retrospective study was to clarify the effect of single tibial artery revascularization on the dorsal and plantar microcirculation of critically ischemic limbs based on skin perfusion pressure (SPP). METHODS AND RESULTS Fifty-seven interventions that only involve...