We evaluated serial enzyme and bilirubin determinations as aids to diagnosis of Epstein-Barr virus-induced infectious mononucleosis (121 cases) and the heterophil-negative mononucleosis-like illness due to cytomegalovirus (33 cases). Laboratory evidence for either type of mononucleosis includes mild to moderate hepatic dysfunction, with aspartate aminotransferase activity increased, but lower t...

We examined the effect of pyridoxal phosphate supplementation on the apparent aspartate aminotransferase (EC 2.6.1.1.) activity of human serum. Supplementation by 25 mol/liter effected an average increase of 16% in the results for kinetic assay. The increase was not the result of increased enzymatic or nonenzymatic blanks, and, within a small range, sample dilution had no significant effect. Pa...

OBJECTIVE To reports changes in plasma levels in professional racing cyclists. METHODS Plasma levels of the intracellular enzymes aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transpeptidase, lactate dehydrogenase, and alkaline phosphatase were measured resting and after exercise in professional cyclists participating in two road races: Vuelta Ciclista a Valencia (800 k...

Serum aspartate aminotransferase isoenzymes were measured in 123 hospital patients with various liver diseases, using a new and simple immunochemical method. Our findings show the usefulness of this determination in estimating the severity of hepatic damage, especially if accompanied by the measurement of other mitochondrial enzymes. During hepatic damage, the cytoplasmic isoenzyme is found in ...

X-ray diffraction studies to 2.8-A resolution have yielded the three-dimensional structure of mitochondrial aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1), an isologous alpha 2 dimer (Mr = 2 x 45,000). The subunits are rich in secondary structure and contain two domains, one of which anchors the coenzyme, pyridoxal 5'-phosphate. Each active site lies betwee...

  The aims of the present study were to determine the best time for egg stripping after ovulation and to study oocyte over-ripening in the Caspian brown trout (Salmo trutta caspius). Eggs were retained in the female abdominal cavity for 40 days post ovulation (DPO). Partial volumes of eggs stripped from 10 individually identified females at 10 days intervals and fertilized with a pool of semen ...

  The aims of the present study were to determine the best time for egg stripping after ovulation and to study oocyte over-ripening in the Caspian brown trout (Salmo trutta caspius). Eggs were retained in the female abdominal cavity for 40 days post ovulation (DPO). Partial volumes of eggs stripped from 10 individually identified females at 10 days intervals and fertilized with a pool of semen ...

In vivo studies with L-[13N]glutamate in the Walker 256 carcinosarcoma implanted under the renal capsule of female Sprague-Dawley rats demonstrate that uptake of glutamate and the rate of incorporation of the nitrogen label from this amino acid into metabolites is slower in the tumor than in nontumorous kidney tissue. Glutamate dehydrogenase, glutaminase, and alanine aminotransferase activities...

Aspartate aminotransferase-to-platelet ratio (APRI), aspartate aminotransferase-to-alanine aminotransferase ratio (AAR), FIB-4, FI, King, Lok, Forns, and FibroIndex scores may be simple and convenient noninvasive diagnostic tests, because they are based on the regular laboratory tests and demographic data. This study aimed to systematically evaluate their diagnostic accuracy for the prediction ...