Novikoff hepatoma ascites cells, grown intraperitoneally in rats, are shown to possess a high-affinity, low-capacity dexamethasone-binding protein. The receptor protein has an intracellular localization and concentration, association constant (Ka), glucocorticoid specificity and nuclear uptake in the presence of dexamethasone comparable with those of the G-protein of rat liver. During therapy o...

Ruthenium red induces the agglutination of ascites hepatoma cells. The agglutination was inhibited by acidic polysaccharides but not by haptenic inhibitors of some plant agglutinins. The agglutination was also abolished by treatment of the cells with a low concentration of papain. Papain digestion causes a decrease in the number of microvilli on the cell surface and the release of carbohydrate-...

Rat-ascites-hepatoma AH 130 cells were incubated at 37°C for 1 hour with bleomycin at the concentration of l~30 #g/ml. Then, DNA polymerase was prepared from the cells. The enzymatic activity was about half of the control value. Thymidine kinase similarly prepared from bleomycin-treated cells, did not show such a marked inhibition in their activities. In vitro addition of bleomycin to the DNA p...

Five permanent tumor cell lines derived originally from either a solid or an ascites biopsy of rat hepatoma exhibited differential sensitivities to bleomycin, Adriamycin, 1-beta-D-arabinofuranosylcytosine, hydroxyurea, 1-trans-(2)-chloroethyl)-3-(4-methoylcyclohexyl)-1-nitrosourea, and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea. The cells were least sensitive to hydroxyurea and 1-beta-D-arabi...