نتایج جستجو برای: arylalkylamine n acetyltransferase aa nat gene

تعداد نتایج: 2032563  

2008
Edith Sim James Sandy Dimitrios Evangelopoulos Elizabeth Fullam Sanjib Bhakta Isaac Westwood Anna Krylova Nathan Lack Martin Noble

Polymorphic Human arylamine N-acetyltransferase (NAT2) inactivates the anti-tubercular drug isoniazid by acetyltransfer from acetylCoA. There are active NAT proteins encoded by homologous genes in mycobacteria including M. tuberculosis, M. bovis BCG, M. smegmatis and M. marinum. Crystallographic structures of NATs from M. smegmatis and M. marinum, as native enzymes and with isoniazid bound shar...

Journal: :The Journal of Experimental Medicine 2004
Sanjib Bhakta Gurdyal S. Besra Anna M. Upton Tanya Parish Carolyn Sholto-Douglas-Vernon Kevin J.C. Gibson Stuart Knutton Siamon Gordon Rosangela P. daSilva Matthew C. Anderton Edith Sim

Mycolic acids represent a major component of the unique cell wall of mycobacteria. Mycolic acid biosynthesis is inhibited by isoniazid, a key frontline antitubercular drug that is inactivated by mycobacterial and human arylamine N-acetyltransferase (NAT). We show that an in-frame deletion of Mycobacterium bovis BCG nat results in delayed entry into log phase, altered morphology, altered cell wa...

Journal: :Journal of bacteriology 2007
Hirokazu Suzuki Yasuo Ohnishi Sueharu Horinouchi

An arylamine N-acetyltransferase (NAT) responsible for the N acetylation of exogenous 3-amino-4-hydroxybenzoic acid in Streptomyces griseus was identified and characterized. This enzyme was distinct from other eukaryotic and bacterial NATs in that it acetylated various 2-aminophenol derivatives more effectively than it acetylated 5-aminosalicylic acid, and thus it may be involved in the metabol...

Journal: :Molecular cancer research : MCR 2003
Paul J Adam Joanne Berry Julie A Loader Kerry L Tyson Graham Craggs Paul Smith Jackie De Belin Graham Steers Francesco Pezzella Kris F Sachsenmeir Alasdair C Stamps Athula Herath Edith Sim Michael J O'Hare Adrian L Harris Jonathan A Terrett

Comparative two-dimensional proteome analysis was used to identify proteins differentially expressed in multiple clinical normal and breast cancer tissues. One protein, the expression of which was elevated in invasive ductal and lobular breast carcinomas when compared with normal breast tissue, was arylamine N-acetyltransferase-1 (NAT-1), a Phase II drug-metabolizing enzyme. NAT-1 overexpressio...

Journal: :Analytical biochemistry 1985
H H Andres A J Klem S M Szabo W W Weber

Simple and sensitive spectrophotometric and radiochemical procedures are described for the assay of acetyl-CoA:arylamine N-acetyltransferase (NAT; EC 2.3.1.5), which catalyzes the reaction acetyl-CoA + arylamine----N-acetylated arylamine + CoASH. The methods are applicable to crude tissue homogenates and blood lysates. The spectrophotometric assay is characterized by two features: (i) NAT activ...

Journal: :General pharmacology 1989
K A Haak G Torres P L Garvey D M Bronstein A K Cho L D Lytle

1. The action of N-2'-chloroethyl-N-ethyl-2-methyl benzylamine (xylamine) on rat pineal gland sympathetic innervation was examined. 2. This alkylating agent caused a concentration-dependent increase in pineal gland N-acetyltransferase (NAT) activity in neurologically intact pineal glands that was suppressed in glands previously subjected to bilateral superior cervical ganglionectomy. 3. Xylamin...

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