Dietary fish oils, which are rich in omega-3 fatty acids, are known to produce a marked lowering of very low density lipoprotein (VLDL) triglyceride concentrations, but they have a less marked effect on low density lipoprotein (LDL) cholesterol. Our previous apolipoprotein (apo) B kinetic studies in miniature pigs demonstrated that conversion of VLDL apo B to LDL apo B accounted for 15% to 20% ...

We describe a method for measuring apolipoprotein (apo) C distribution between apo B-containing lipoprotein (apo B-LP) and non-apo B-LP. The procedure requires the precipitation of apo B-LP, the redissolution of the pellet, and the quantification of C peptides in the redissolved pellet. The ratio of apo C in non-apo B-LP to apo C in apo B-LP has been calculated for both CII and CII (R-CII and R...

The purpose of this study was to determine the effect of chronic (1 wk) feeding of dietary triacylglycerol (TG) of varying fatty acid composition on small intestinal and hepatic apolipoprotein expression, as well as serum lipid and apolipoprotein concentrations, in newborn swine. Two-day-old female swine were fed one of three diets by gavage with the following lipid composition: medium-chain TG...

Endogenous apolipoprotein E in VLDL is poorly expressed in receptor binding processes. Yet catabolism ofVLDL-remnants by cellular receptors depends on functional apo E molecules. To better understand remnant catabolism phenomena, we determined the metabolism of VLDL and post-lipolysis VLDL by cultured cells. Partial lipolysis was achieved by incubation of VLDL with lipoprotein lipase in vitro (...

An inherited metabolic disorder in a strain of New Zealand White rabbits, characterized by marked hypercholesterolemia (394 +/- 100 mg/dl), with moderately elevated or normal triglyceride levels is described. Low density lipoprotein (LDL), intermediate density lipoprotein (IDL) and very low density lipoprotein (VLDL) cholesterol levels were increased. VLDL and IDL, and to a lesser extent LDL, h...

The concentration of triglyceride-rich lipoproteins containing apolipoprotein (apo) B-48 (chylomicrons) and apo B-100 (very low density lipoproteins) was measured in blood plasma of healthy young men after an ordinary meal containing one-third of daily energy and fat. Plasma obtained in the postabsorptive state and at intervals up to 12 hr after the meal was subjected to immunoaffinity chromato...

Gene expression et' apolipeproteins (apo) A-l, A-IV, and B, the predominant pretein components of chylomicr{}ns, was in\estigated in the residual ileum after a massive small bowel resection in rats. A Northern blot analysis showed that the apo A-IV mRNA leyel, but not the apo A-I and B mRNA leyels, in the ileum was significantiy higher in the resected rats than in the sham-operated rats 24h and...

BackgroundThe aim of this study was to evaluate the lipid abnormalities associated with different stages of albuminuria and also examine role of ApoB lipoprotein as a predictor of early diabetic nephropathy in type 2 diabetic patients. Methods and ResultsA total 100 diabetic patients (58 men and 42 women) with mean age group 59.15±10.4 years were studied. Normoalbuminurea (n=36), microalbuminur...

BACKGROUND Few studies have examined the changes in lipoproteins over time and how inflammation is associated with lipoprotein concentrations among patients with end-stage renal disease on dialysis. One possible explanation for the association of low LDL cholesterol concentration and adverse outcomes is that inflammation reduces selected apolipoprotein concentrations. METHODS Serum samples we...

The C apolipoproteins are normally transferred to high density llpoprotelns (HDL) after lipolysis of very low density llpoproteln (VLDL) trlglycerlde. In previous studies, a loss of plasma C apolipoproteins was documented after heparin-induced lipolysis In hypertriglyceridemic subjects. The present studies were designed to determine If this decline In plasma C apolipoproteins was due to their c...