semen from six adult male black bengal goats (capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. each collected samples was divided into two parts: one part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚c) and equilibrated for freezing.in both cases, semen was dilu...

Background Although several studies confirmed positive effects of fish oil on semen quality, the antioxidant status in omega-3 supplemented diets of several studies is a fuzzy point. The aim of this study was to investigate the effect of dietary fish oil and (or) vitamin E supplementation on cryopreserved sperm in Mehraban rams. MaterialsAndMethods Sixteen fertile rams were randomly allotted to...

Introduction: Cryopreservation of semen is routinely used in a variety of circumstances including before assisted reproduction treatments, pre- radiation or chemotherapy treatment and etc. The aim of this study was to compare the effect of Butylated hydroxytoluene (BHT) and Glutathione supplemented cryopreservation medium on sperm parameters and amount of DNA fragmentation during the freeze-tha...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

Aim of the study: to evaluate the functional parameters of bovine spermatozoa before and after separation by single layer centrifugation using Bovipure gradient. Material and Methods: Frozen semen from 8 bulls was used in this study. Thawed pellets were subjected to the separation process through gradient media according to the manufacturer’s instructions. Qualitative assessment of semen was pe...

The aim of this study was to investigate the effects of catalase and pre-freezing equilibration during ram sperm cryopreservation on motility and membrane and acrosomal integrity of frozen-thawed semen, as well as conception rate following laparoscopic timedinsemination. Semen was collected from four mature Dorper rams, pooled and diluted in Tris egg-yolk extender basic solution (CON), or this ...

The integrity of sperm progesterone (P4) receptor(s) and its response to steroid stimulation might be crucial for the maintenance of sperm fertilizing ability after cryopreservation. The aim of the current investigation was to study the effect of cryo-procedures on canine sperm P4 receptor(s). In addition, alteration of P4 receptor(s) at the molecular level and their functional integrity follow...

BACKGROUND Although routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men. ...